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Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages
The bacterium Escherichia coli exhibits remarkable genomic and phenotypic variation, with some pathogenic strains having evolved to survive and even replicate in the harsh intra-macrophage environment. The rate and effects of mutations that can cause pathoadaptation are key determinants of the pace...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709186/ https://www.ncbi.nlm.nih.gov/pubmed/26752723 http://dx.doi.org/10.1371/journal.pone.0146123 |
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author | Azevedo, M. Sousa, A. Moura de Sousa, J. Thompson, J. A. Proença, J. T. Gordo, I. |
author_facet | Azevedo, M. Sousa, A. Moura de Sousa, J. Thompson, J. A. Proença, J. T. Gordo, I. |
author_sort | Azevedo, M. |
collection | PubMed |
description | The bacterium Escherichia coli exhibits remarkable genomic and phenotypic variation, with some pathogenic strains having evolved to survive and even replicate in the harsh intra-macrophage environment. The rate and effects of mutations that can cause pathoadaptation are key determinants of the pace at which E. coli can colonize such niches and become pathogenic. We used experimental evolution to determine the speed and evolutionary paths undertaken by a commensal strain of E. coli when adapting to intracellular life. We estimated the acquisition of pathoadaptive mutations at a rate of 10(−6) per genome per generation, resulting in the fixation of more virulent strains in less than a hundred generations. Whole genome sequencing of independently evolved clones showed that the main targets of intracellular adaptation involved loss of function mutations in genes implicated in the assembly of the lipopolysaccharide core, iron metabolism and di- and tri-peptide transport, namely rfaI, fhuA and tppB, respectively. We found a substantial amount of antagonistic pleiotropy in evolved populations, as well as metabolic trade-offs, commonly found in intracellular bacteria with reduced genome sizes. Overall, the low levels of clonal interference detected indicate that the first steps of the transition of a commensal E. coli into intracellular pathogens are dominated by a few pathoadaptive mutations with very strong effects. |
format | Online Article Text |
id | pubmed-4709186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47091862016-01-15 Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages Azevedo, M. Sousa, A. Moura de Sousa, J. Thompson, J. A. Proença, J. T. Gordo, I. PLoS One Research Article The bacterium Escherichia coli exhibits remarkable genomic and phenotypic variation, with some pathogenic strains having evolved to survive and even replicate in the harsh intra-macrophage environment. The rate and effects of mutations that can cause pathoadaptation are key determinants of the pace at which E. coli can colonize such niches and become pathogenic. We used experimental evolution to determine the speed and evolutionary paths undertaken by a commensal strain of E. coli when adapting to intracellular life. We estimated the acquisition of pathoadaptive mutations at a rate of 10(−6) per genome per generation, resulting in the fixation of more virulent strains in less than a hundred generations. Whole genome sequencing of independently evolved clones showed that the main targets of intracellular adaptation involved loss of function mutations in genes implicated in the assembly of the lipopolysaccharide core, iron metabolism and di- and tri-peptide transport, namely rfaI, fhuA and tppB, respectively. We found a substantial amount of antagonistic pleiotropy in evolved populations, as well as metabolic trade-offs, commonly found in intracellular bacteria with reduced genome sizes. Overall, the low levels of clonal interference detected indicate that the first steps of the transition of a commensal E. coli into intracellular pathogens are dominated by a few pathoadaptive mutations with very strong effects. Public Library of Science 2016-01-11 /pmc/articles/PMC4709186/ /pubmed/26752723 http://dx.doi.org/10.1371/journal.pone.0146123 Text en © 2016 Azevedo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Azevedo, M. Sousa, A. Moura de Sousa, J. Thompson, J. A. Proença, J. T. Gordo, I. Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title | Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title_full | Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title_fullStr | Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title_full_unstemmed | Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title_short | Trade-Offs of Escherichia coli Adaptation to an Intracellular Lifestyle in Macrophages |
title_sort | trade-offs of escherichia coli adaptation to an intracellular lifestyle in macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709186/ https://www.ncbi.nlm.nih.gov/pubmed/26752723 http://dx.doi.org/10.1371/journal.pone.0146123 |
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