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Effect of methotrexate on rostral migratory stream in newborn rats

Two-day-old rats were treated with subcutaneous injections of methotrexate (MTX) 5 mg/kg and 150 mg/kg, and their rostral migratory streams (RMS) were examined time-dependently. MTX treatment increased pyknotic and TUNEL-positive cells and decreased mitotic and phospho-Histone H3-positive cells at a...

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Autores principales: HIRAKO, Ayano, SUN, Jin, FURUKAWA, Satoshi, TAKEUCHI, Takashi, SUGIYAMA, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710711/
https://www.ncbi.nlm.nih.gov/pubmed/26136044
http://dx.doi.org/10.1292/jvms.15-0280
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author HIRAKO, Ayano
SUN, Jin
FURUKAWA, Satoshi
TAKEUCHI, Takashi
SUGIYAMA, Akihiko
author_facet HIRAKO, Ayano
SUN, Jin
FURUKAWA, Satoshi
TAKEUCHI, Takashi
SUGIYAMA, Akihiko
author_sort HIRAKO, Ayano
collection PubMed
description Two-day-old rats were treated with subcutaneous injections of methotrexate (MTX) 5 mg/kg and 150 mg/kg, and their rostral migratory streams (RMS) were examined time-dependently. MTX treatment increased pyknotic and TUNEL-positive cells and decreased mitotic and phospho-Histone H3-positive cells at almost all time points in the vertical arm, elbow and horizontal arm regions of the RMS. There were more TUNEL-positive cells ratio in the MTX 150 mg/kg group than in the MTX 5 mg/kg group. Treatment with MTX 150 mg/kg decreased the cellularity in the vertical arm region on Postnatal day (PD) 4, but that with the MTX 5 mg/kg did not. TUNEL-positive cells ratio was the highest in the vertical arm region, followed by elbow and horizontal regions in both MTX-treated groups. TUNEL-positive cells ratio in the vertical arm and elbow regions reached their peaks on PD 4 in both MTX-treated groups, and both MTX-treatments significantly decreased Phospho-Histone H3-positive cells ratio on PDs 2.5 and 3 in the vertical arm, elbow and horizontal arm regions. The phospho-Histone H3-positive cells ratio in the vertical arm region recovered on PD4 in the MTX 150 mg/kg group. These findings suggested that RMS required a great amount of folic acid on PD 2 and that the folic acid-requirement differed depending on the anatomical region of the RMS. To our knowledge, this is the first report demonstrating the effect of MTX on the RMS and the necessity of the folic acid metabolism on RMS development in newborn rats.
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spelling pubmed-47107112016-01-14 Effect of methotrexate on rostral migratory stream in newborn rats HIRAKO, Ayano SUN, Jin FURUKAWA, Satoshi TAKEUCHI, Takashi SUGIYAMA, Akihiko J Vet Med Sci Pathology Two-day-old rats were treated with subcutaneous injections of methotrexate (MTX) 5 mg/kg and 150 mg/kg, and their rostral migratory streams (RMS) were examined time-dependently. MTX treatment increased pyknotic and TUNEL-positive cells and decreased mitotic and phospho-Histone H3-positive cells at almost all time points in the vertical arm, elbow and horizontal arm regions of the RMS. There were more TUNEL-positive cells ratio in the MTX 150 mg/kg group than in the MTX 5 mg/kg group. Treatment with MTX 150 mg/kg decreased the cellularity in the vertical arm region on Postnatal day (PD) 4, but that with the MTX 5 mg/kg did not. TUNEL-positive cells ratio was the highest in the vertical arm region, followed by elbow and horizontal regions in both MTX-treated groups. TUNEL-positive cells ratio in the vertical arm and elbow regions reached their peaks on PD 4 in both MTX-treated groups, and both MTX-treatments significantly decreased Phospho-Histone H3-positive cells ratio on PDs 2.5 and 3 in the vertical arm, elbow and horizontal arm regions. The phospho-Histone H3-positive cells ratio in the vertical arm region recovered on PD4 in the MTX 150 mg/kg group. These findings suggested that RMS required a great amount of folic acid on PD 2 and that the folic acid-requirement differed depending on the anatomical region of the RMS. To our knowledge, this is the first report demonstrating the effect of MTX on the RMS and the necessity of the folic acid metabolism on RMS development in newborn rats. The Japanese Society of Veterinary Science 2015-07-02 2015-12 /pmc/articles/PMC4710711/ /pubmed/26136044 http://dx.doi.org/10.1292/jvms.15-0280 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Pathology
HIRAKO, Ayano
SUN, Jin
FURUKAWA, Satoshi
TAKEUCHI, Takashi
SUGIYAMA, Akihiko
Effect of methotrexate on rostral migratory stream in newborn rats
title Effect of methotrexate on rostral migratory stream in newborn rats
title_full Effect of methotrexate on rostral migratory stream in newborn rats
title_fullStr Effect of methotrexate on rostral migratory stream in newborn rats
title_full_unstemmed Effect of methotrexate on rostral migratory stream in newborn rats
title_short Effect of methotrexate on rostral migratory stream in newborn rats
title_sort effect of methotrexate on rostral migratory stream in newborn rats
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710711/
https://www.ncbi.nlm.nih.gov/pubmed/26136044
http://dx.doi.org/10.1292/jvms.15-0280
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