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An Improved Quantitative Real-Time PCR Assay for the Enumeration of Heterosigma akashiwo (Raphidophyceae) Cysts Using a DNA Debris Removal Method and a Cyst-Based Standard Curve

The identification and quantification of Heterosigma akashiwo cysts in sediments by light microscopy can be difficult due to the small size and morphology of the cysts, which are often indistinguishable from those of other types of algae. Quantitative real-time PCR (qPCR) based assays represent a po...

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Detalles Bibliográficos
Autores principales: Kim, Joo-Hwan, Kim, Jin Ho, Wang, Pengbin, Park, Bum Soo, Han, Myung-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712154/
https://www.ncbi.nlm.nih.gov/pubmed/26741648
http://dx.doi.org/10.1371/journal.pone.0145712