Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison with Sanger sequencing and ARMS-Scorpion real-time PCR

OBJECTIVE: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations detection in formalin-fixed paraffin-embedded (FFPE) colorectal cancer (CRC) specimens, with comparison of Sanger sequencing and ARMS-Scorpion real-time PCR. SETTI...

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Detalles Bibliográficos
Autores principales: Gao, Jie, Wu, Huanwen, Wang, Li, Zhang, Hui, Duan, Huanli, Lu, Junliang, Liang, Zhiyong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2016
Materias:
Diagnostics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716245/
https://www.ncbi.nlm.nih.gov/pubmed/26747035
http://dx.doi.org/10.1136/bmjopen-2015-009532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716245/
https://www.ncbi.nlm.nih.gov/pubmed/26747035
http://dx.doi.org/10.1136/bmjopen-2015-009532

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