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Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions
Excessive expansion of the transit-amplifying (TA) cell compartment is a distinct morphological characteristic of psoriatic epidermal hyperplasia. In order to examine the activation of basal stem cells and how they replenish such an enlarged compartment of TA cells in psoriatic epidermis, we utilize...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716788/ https://www.ncbi.nlm.nih.gov/pubmed/26707630 http://dx.doi.org/10.3892/ijmm.2015.2445 |
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author | JIA, HAI-YAN SHI, YING LUO, LONG-FEI JIANG, GUAN ZHOU, QIONG XU, SHI-ZHENG LEI, TIE-CHI |
author_facet | JIA, HAI-YAN SHI, YING LUO, LONG-FEI JIANG, GUAN ZHOU, QIONG XU, SHI-ZHENG LEI, TIE-CHI |
author_sort | JIA, HAI-YAN |
collection | PubMed |
description | Excessive expansion of the transit-amplifying (TA) cell compartment is a distinct morphological characteristic of psoriatic epidermal hyperplasia. In order to examine the activation of basal stem cells and how they replenish such an enlarged compartment of TA cells in psoriatic epidermis, we utilized a BrdU labeling method to monitor mitotic stem cells in a mouse model of psoriasiform dermatitis, which was induced by imiquimod. Our results showed that perpendicular and parallel cell division characteristics of dividing stem cells existed in the inflamed epidermis. When we analyzed template-DNA strand segregation in trypsin-dissociated human psoriatic keratinocytes using BrdU pulse-chase labeling, we found that the percentage of asymmetric segregation of BrdU was significantly increased in the cell pairs of psoriatic epidermal cells compared with normal epidermal cells. Furthermore, we also examined the effects of both interleukin (IL)-17A and IL-22 cytokines on the differentiation status of cultured human keratinocytes. The results indicated that both cytokines had synergistic effects on passage-one epidermal cell sheets derived from skin explants and also on cultured keratinocytes, were involved in the maintenance of the undifferentiated stem cell phenotype, and these results suggest an efficient mechanism for preventing the premature loss of basal stem-cell pools in the pro-inflammatory cytokine-enriched milieu of the psoriatic epidermis. Our findings suggest that inhibition of hyperactive stem cells represents a potential therapeutic target to combat recalcitrant epidermal hyperplasia in psoriasis. |
format | Online Article Text |
id | pubmed-4716788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-47167882016-01-22 Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions JIA, HAI-YAN SHI, YING LUO, LONG-FEI JIANG, GUAN ZHOU, QIONG XU, SHI-ZHENG LEI, TIE-CHI Int J Mol Med Articles Excessive expansion of the transit-amplifying (TA) cell compartment is a distinct morphological characteristic of psoriatic epidermal hyperplasia. In order to examine the activation of basal stem cells and how they replenish such an enlarged compartment of TA cells in psoriatic epidermis, we utilized a BrdU labeling method to monitor mitotic stem cells in a mouse model of psoriasiform dermatitis, which was induced by imiquimod. Our results showed that perpendicular and parallel cell division characteristics of dividing stem cells existed in the inflamed epidermis. When we analyzed template-DNA strand segregation in trypsin-dissociated human psoriatic keratinocytes using BrdU pulse-chase labeling, we found that the percentage of asymmetric segregation of BrdU was significantly increased in the cell pairs of psoriatic epidermal cells compared with normal epidermal cells. Furthermore, we also examined the effects of both interleukin (IL)-17A and IL-22 cytokines on the differentiation status of cultured human keratinocytes. The results indicated that both cytokines had synergistic effects on passage-one epidermal cell sheets derived from skin explants and also on cultured keratinocytes, were involved in the maintenance of the undifferentiated stem cell phenotype, and these results suggest an efficient mechanism for preventing the premature loss of basal stem-cell pools in the pro-inflammatory cytokine-enriched milieu of the psoriatic epidermis. Our findings suggest that inhibition of hyperactive stem cells represents a potential therapeutic target to combat recalcitrant epidermal hyperplasia in psoriasis. D.A. Spandidos 2016-02 2015-12-23 /pmc/articles/PMC4716788/ /pubmed/26707630 http://dx.doi.org/10.3892/ijmm.2015.2445 Text en Copyright: © Jia et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles JIA, HAI-YAN SHI, YING LUO, LONG-FEI JIANG, GUAN ZHOU, QIONG XU, SHI-ZHENG LEI, TIE-CHI Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title | Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title_full | Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title_fullStr | Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title_full_unstemmed | Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title_short | Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
title_sort | asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716788/ https://www.ncbi.nlm.nih.gov/pubmed/26707630 http://dx.doi.org/10.3892/ijmm.2015.2445 |
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