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Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the mat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Biomedical
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/ https://www.ncbi.nlm.nih.gov/pubmed/26656001 http://dx.doi.org/10.1016/j.mimet.2015.12.004 |