Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the mat...

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Detalles Bibliográficos
Autores principales: Larrouy-Maumus, Gerald, Clements, Abigail, Filloux, Alain, McCarthy, Ronan R., Mostowy, Serge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Biomedical 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/
https://www.ncbi.nlm.nih.gov/pubmed/26656001
http://dx.doi.org/10.1016/j.mimet.2015.12.004
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author Larrouy-Maumus, Gerald
Clements, Abigail
Filloux, Alain
McCarthy, Ronan R.
Mostowy, Serge
author_facet Larrouy-Maumus, Gerald
Clements, Abigail
Filloux, Alain
McCarthy, Ronan R.
Mostowy, Serge
author_sort Larrouy-Maumus, Gerald
collection PubMed
description The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry.
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spelling pubmed-47171202016-02-12 Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry Larrouy-Maumus, Gerald Clements, Abigail Filloux, Alain McCarthy, Ronan R. Mostowy, Serge J Microbiol Methods Article The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the matrix combined with MALDI-TOF mass spectrometry. Elsevier Biomedical 2016-01 /pmc/articles/PMC4717120/ /pubmed/26656001 http://dx.doi.org/10.1016/j.mimet.2015.12.004 Text en © 2015 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Larrouy-Maumus, Gerald
Clements, Abigail
Filloux, Alain
McCarthy, Ronan R.
Mostowy, Serge
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_full Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_fullStr Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_full_unstemmed Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_short Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry
title_sort direct detection of lipid a on intact gram-negative bacteria by maldi-tof mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/
https://www.ncbi.nlm.nih.gov/pubmed/26656001
http://dx.doi.org/10.1016/j.mimet.2015.12.004
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