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Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry

The purification and characterization of Gram-negative bacterial lipid A is tedious and time-consuming. Herein we report a rapid and sensitive method to identify lipid A directly on intact bacteria without any chemical treatment or purification, using an atypical solvent system to solubilize the mat...

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Detalles Bibliográficos
Autores principales:	Larrouy-Maumus, Gerald, Clements, Abigail, Filloux, Alain, McCarthy, Ronan R., Mostowy, Serge
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier Biomedical 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717120/ https://www.ncbi.nlm.nih.gov/pubmed/26656001 http://dx.doi.org/10.1016/j.mimet.2015.12.004

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