Evaluation of fluoropyruvate as nucleophile in reactions catalysed by N-acetyl neuraminic acid lyase variants: scope, limitations and stereoselectivity

The catalysis of reactions involving fluoropyruvate as donor by N-acetyl neuraminic acid lyase (NAL) variants was investigated. Under kinetic control, the wild-type enzyme catalysed the reaction between fluoropyruvate and N-acetyl mannosamine to give a 90 : 10 ratio of the (3R,4R)- and (3S,4R)-confi...

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Detalles Bibliográficos
Autores principales: Stockwell, Jennifer, Daniels, Adam D., Windle, Claire L., Harman, Thomas A., Woodhall, Thomas, Lebl, Tomas, Trinh, Chi H., Mulholland, Keith, Pearson, Arwen R., Berry, Alan, Nelson, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2016
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717870/
https://www.ncbi.nlm.nih.gov/pubmed/26537532
http://dx.doi.org/10.1039/c5ob02037a

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717870/
https://www.ncbi.nlm.nih.gov/pubmed/26537532
http://dx.doi.org/10.1039/c5ob02037a

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