Cargando…
Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation
Crk and CrkL are noncatalytic adaptor proteins necessary for the formation of neuromuscular synapses which function downstream of muscle-specific kinase (MuSK), a receptor tyrosine kinase expressed in skeletal muscle, and the MuSK binding protein Dok-7. How Crk/CrkL regulate neuromuscular endplate f...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719301/ https://www.ncbi.nlm.nih.gov/pubmed/26527617 http://dx.doi.org/10.1128/MCB.00775-15 |
_version_ | 1782410910569070592 |
---|---|
author | Hallock, Peter T. Chin, Sherry Blais, Steven Neubert, Thomas A. Glass, David J. |
author_facet | Hallock, Peter T. Chin, Sherry Blais, Steven Neubert, Thomas A. Glass, David J. |
author_sort | Hallock, Peter T. |
collection | PubMed |
description | Crk and CrkL are noncatalytic adaptor proteins necessary for the formation of neuromuscular synapses which function downstream of muscle-specific kinase (MuSK), a receptor tyrosine kinase expressed in skeletal muscle, and the MuSK binding protein Dok-7. How Crk/CrkL regulate neuromuscular endplate formation is not known. To better understand the roles of Crk/CrkL, we identified CrkL binding proteins using mass spectrometry and have identified Sorbs1 and Sorbs2 as two functionally redundant proteins that associate with the initiating MuSK/Dok-7/Crk/CrkL complex, regulate acetylcholine receptor (AChR) clustering in vitro, and are localized at synapses in vivo. |
format | Online Article Text |
id | pubmed-4719301 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47193012016-02-12 Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation Hallock, Peter T. Chin, Sherry Blais, Steven Neubert, Thomas A. Glass, David J. Mol Cell Biol Articles Crk and CrkL are noncatalytic adaptor proteins necessary for the formation of neuromuscular synapses which function downstream of muscle-specific kinase (MuSK), a receptor tyrosine kinase expressed in skeletal muscle, and the MuSK binding protein Dok-7. How Crk/CrkL regulate neuromuscular endplate formation is not known. To better understand the roles of Crk/CrkL, we identified CrkL binding proteins using mass spectrometry and have identified Sorbs1 and Sorbs2 as two functionally redundant proteins that associate with the initiating MuSK/Dok-7/Crk/CrkL complex, regulate acetylcholine receptor (AChR) clustering in vitro, and are localized at synapses in vivo. American Society for Microbiology 2016-01-04 /pmc/articles/PMC4719301/ /pubmed/26527617 http://dx.doi.org/10.1128/MCB.00775-15 Text en Copyright © 2016 Hallock et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Articles Hallock, Peter T. Chin, Sherry Blais, Steven Neubert, Thomas A. Glass, David J. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title | Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title_full | Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title_fullStr | Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title_full_unstemmed | Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title_short | Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation |
title_sort | sorbs1 and -2 interact with crkl and are required for acetylcholine receptor cluster formation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719301/ https://www.ncbi.nlm.nih.gov/pubmed/26527617 http://dx.doi.org/10.1128/MCB.00775-15 |
work_keys_str_mv | AT hallockpetert sorbs1and2interactwithcrklandarerequiredforacetylcholinereceptorclusterformation AT chinsherry sorbs1and2interactwithcrklandarerequiredforacetylcholinereceptorclusterformation AT blaissteven sorbs1and2interactwithcrklandarerequiredforacetylcholinereceptorclusterformation AT neubertthomasa sorbs1and2interactwithcrklandarerequiredforacetylcholinereceptorclusterformation AT glassdavidj sorbs1and2interactwithcrklandarerequiredforacetylcholinereceptorclusterformation |