Cargando…

Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet

INTRODUCTION: Pulsatile hyperglycaemia resulting in oxidative stress may play an important role in the development of macrovascular complications. We investigated the effects of sustained vs. pulsatile hyperglycaemia in insulin resistant rats on markers of oxidative stress, enzyme expression and glu...

Descripción completa

Detalles Bibliográficos
Autores principales: Rakipovski, Günaj, Lykkesfeldt, Jens, Raun, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720376/
https://www.ncbi.nlm.nih.gov/pubmed/26790104
http://dx.doi.org/10.1371/journal.pone.0147412
_version_ 1782411071610421248
author Rakipovski, Günaj
Lykkesfeldt, Jens
Raun, Kirsten
author_facet Rakipovski, Günaj
Lykkesfeldt, Jens
Raun, Kirsten
author_sort Rakipovski, Günaj
collection PubMed
description INTRODUCTION: Pulsatile hyperglycaemia resulting in oxidative stress may play an important role in the development of macrovascular complications. We investigated the effects of sustained vs. pulsatile hyperglycaemia in insulin resistant rats on markers of oxidative stress, enzyme expression and glucose metabolism in liver and aorta. We hypothesized that liver’s ability to regulate the glucose homeostasis under varying states of hyperglycaemia may indirectly affect oxidative stress status in aorta despite the amount of glucose challenged with. METHODS: Animals were infused with sustained high (SHG), low (SLG), pulsatile (PLG) glucose or saline (VEH) for 96 h. Oxidative stress status and key regulators of glucose metabolism in liver and aorta were investigated. RESULTS: Similar response in plasma lipid oxidation was observed in PLG as in SHG. Likewise, in aorta, PLG and SHG displayed increased expression of glucose transporter 1 (GLUT1), gp-91(PHOX) and super oxide dismutase (SOD), while only the PLG group showed increased accumulation of oxidative stress and oxidised low density lipoprotein (oxLDL) in aorta. CONCLUSION: Pulsatile hyperglycaemia induced relatively higher levels of oxidative stress systemically and in aorta in particular than overt sustained hyperglycaemia thus supporting the clinical observations that pulsatile hyperglycaemia is an independent risk factor for diabetes related macrovascular complications.
format Online
Article
Text
id pubmed-4720376
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47203762016-01-30 Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet Rakipovski, Günaj Lykkesfeldt, Jens Raun, Kirsten PLoS One Research Article INTRODUCTION: Pulsatile hyperglycaemia resulting in oxidative stress may play an important role in the development of macrovascular complications. We investigated the effects of sustained vs. pulsatile hyperglycaemia in insulin resistant rats on markers of oxidative stress, enzyme expression and glucose metabolism in liver and aorta. We hypothesized that liver’s ability to regulate the glucose homeostasis under varying states of hyperglycaemia may indirectly affect oxidative stress status in aorta despite the amount of glucose challenged with. METHODS: Animals were infused with sustained high (SHG), low (SLG), pulsatile (PLG) glucose or saline (VEH) for 96 h. Oxidative stress status and key regulators of glucose metabolism in liver and aorta were investigated. RESULTS: Similar response in plasma lipid oxidation was observed in PLG as in SHG. Likewise, in aorta, PLG and SHG displayed increased expression of glucose transporter 1 (GLUT1), gp-91(PHOX) and super oxide dismutase (SOD), while only the PLG group showed increased accumulation of oxidative stress and oxidised low density lipoprotein (oxLDL) in aorta. CONCLUSION: Pulsatile hyperglycaemia induced relatively higher levels of oxidative stress systemically and in aorta in particular than overt sustained hyperglycaemia thus supporting the clinical observations that pulsatile hyperglycaemia is an independent risk factor for diabetes related macrovascular complications. Public Library of Science 2016-01-20 /pmc/articles/PMC4720376/ /pubmed/26790104 http://dx.doi.org/10.1371/journal.pone.0147412 Text en © 2016 Rakipovski et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rakipovski, Günaj
Lykkesfeldt, Jens
Raun, Kirsten
Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title_full Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title_fullStr Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title_full_unstemmed Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title_short Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet
title_sort pulsatile hyperglycaemia induces vascular oxidative stress and glut 1 expression more potently than sustained hyperglycaemia in rats on high fat diet
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720376/
https://www.ncbi.nlm.nih.gov/pubmed/26790104
http://dx.doi.org/10.1371/journal.pone.0147412
work_keys_str_mv AT rakipovskigunaj pulsatilehyperglycaemiainducesvascularoxidativestressandglut1expressionmorepotentlythansustainedhyperglycaemiainratsonhighfatdiet
AT lykkesfeldtjens pulsatilehyperglycaemiainducesvascularoxidativestressandglut1expressionmorepotentlythansustainedhyperglycaemiainratsonhighfatdiet
AT raunkirsten pulsatilehyperglycaemiainducesvascularoxidativestressandglut1expressionmorepotentlythansustainedhyperglycaemiainratsonhighfatdiet