Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis

Glucose sensitivity and pH and thermal stabilities of Trichoderma reesei Cel1A (Bgl II) were improved by site-directed mutagenesis of only two amino acid residues (L167W or P172L) at the entrance of the active site. The Cel1A mutant showed high glucose tolerance (50% of inhibitory concentration = 65...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Boyang, Amano, Yoshihiko, Nozaki, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720395/
https://www.ncbi.nlm.nih.gov/pubmed/26790148
http://dx.doi.org/10.1371/journal.pone.0147301

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720395/
https://www.ncbi.nlm.nih.gov/pubmed/26790148
http://dx.doi.org/10.1371/journal.pone.0147301

Ejemplares similares