Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions

CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement can be exploited to specifically target single-nucleotide heterozygous muta...

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Detalles Bibliográficos
Autores principales: Li, Yi, Mendiratta, Saurabh, Ehrhardt, Kristina, Kashyap, Neha, White, Michael A., Bleris, Leonidas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720446/
https://www.ncbi.nlm.nih.gov/pubmed/26788852
http://dx.doi.org/10.1371/journal.pone.0144970

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720446/
https://www.ncbi.nlm.nih.gov/pubmed/26788852
http://dx.doi.org/10.1371/journal.pone.0144970

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