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Exploiting the CRISPR/Cas9 PAM Constraint for Single-Nucleotide Resolution Interventions
CRISPR/Cas9 is an enabling RNA-guided technology for genome targeting and engineering. An acute DNA binding constraint of the Cas9 protein is the Protospacer Adjacent Motif (PAM). Here we demonstrate that the PAM requirement can be exploited to specifically target single-nucleotide heterozygous muta...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720446/ https://www.ncbi.nlm.nih.gov/pubmed/26788852 http://dx.doi.org/10.1371/journal.pone.0144970 |