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Site-Directed Mutagenesis to Improve Sensitivity of a Synthetic Two-Component Signaling System

Two-component signaling (2CS) systems enable bacterial cells to respond to changes in their local environment, often using a membrane-bound sensor protein and a cytoplasmic responder protein to regulate gene expression. Previous work has shown that Escherichia coli’s natural EnvZ/OmpR 2CS could be m...

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Detalles Bibliográficos
Autores principales: Olshefsky, Audrey, Shehata, Laila, Kuldell, Natalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723039/
https://www.ncbi.nlm.nih.gov/pubmed/26799494
http://dx.doi.org/10.1371/journal.pone.0147494