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Comparison of Parasite Burden Using Real-Time Polymerase Chain Reaction Assay and Limiting Dilution Assay in Leishmania major Infected Mouse

BACKGROUND: Limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of Leishmania infection. Nowadays, real-time PCR is being increasingly applied to quantify infectious agents. In the present study, a real-time PCR assay was deve...

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Detalles Bibliográficos
Autores principales: GHOTLOO, Somayeh, HAJI MOLLAHOSEINI, Mostafa, NAJAFI, Ali, YEGANEH, Farshid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4724833/
https://www.ncbi.nlm.nih.gov/pubmed/26811723