Cargando…
An efficient full-length cDNA amplification strategy based on bioinformatics technology and multiplexed PCR methods
A novel strategy for amplification full-length cDNA and promoter sequences has been developed using bioinformatics technology and multiplexed PCR methods in this study. The amplification of 3′ ends of cDNA is performed according to the modified classic 3′ RACE techniques, therein the more efficient...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725349/ https://www.ncbi.nlm.nih.gov/pubmed/26758040 http://dx.doi.org/10.1038/srep19420 |