Two-Photon Excitation STED Microscopy with Time-Gated Detection

We report on a novel two-photon excitation stimulated emission depletion (2PE-STED) microscope based on time-gated detection. The time-gated detection allows for the effective silencing of the fluorophores using moderate stimulated emission beam intensity. This opens the possibility of implementing...

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Detalles Bibliográficos
Autores principales: Coto Hernández, Iván, Castello, Marco, Lanzanò, Luca, d’Amora, Marta, Bianchini, Paolo, Diaspro, Alberto, Vicidomini, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725939/
https://www.ncbi.nlm.nih.gov/pubmed/26757892
http://dx.doi.org/10.1038/srep19419

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725939/
https://www.ncbi.nlm.nih.gov/pubmed/26757892
http://dx.doi.org/10.1038/srep19419

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