A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance

Antibiotic resistance in clinically important bacteria can be mediated by proteins that physically associate with the drug target and act to protect it from the inhibitory effects of an antibiotic. We present here the first detailed structural characterization of such a target protection mechanism m...

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Autores principales: Tomlinson, Jennifer H., Thompson, Gary S., Kalverda, Arnout P., Zhuravleva, Anastasia, O’Neill, Alex J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725979/
https://www.ncbi.nlm.nih.gov/pubmed/26781961
http://dx.doi.org/10.1038/srep19524
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author Tomlinson, Jennifer H.
Thompson, Gary S.
Kalverda, Arnout P.
Zhuravleva, Anastasia
O’Neill, Alex J.
author_facet Tomlinson, Jennifer H.
Thompson, Gary S.
Kalverda, Arnout P.
Zhuravleva, Anastasia
O’Neill, Alex J.
author_sort Tomlinson, Jennifer H.
collection PubMed
description Antibiotic resistance in clinically important bacteria can be mediated by proteins that physically associate with the drug target and act to protect it from the inhibitory effects of an antibiotic. We present here the first detailed structural characterization of such a target protection mechanism mediated through a protein-protein interaction, revealing the architecture of the complex formed between the FusB fusidic acid resistance protein and the drug target (EF-G) it acts to protect. Binding of FusB to EF-G induces conformational and dynamic changes in the latter, shedding light on the molecular mechanism of fusidic acid resistance.
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spelling pubmed-47259792016-01-28 A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance Tomlinson, Jennifer H. Thompson, Gary S. Kalverda, Arnout P. Zhuravleva, Anastasia O’Neill, Alex J. Sci Rep Article Antibiotic resistance in clinically important bacteria can be mediated by proteins that physically associate with the drug target and act to protect it from the inhibitory effects of an antibiotic. We present here the first detailed structural characterization of such a target protection mechanism mediated through a protein-protein interaction, revealing the architecture of the complex formed between the FusB fusidic acid resistance protein and the drug target (EF-G) it acts to protect. Binding of FusB to EF-G induces conformational and dynamic changes in the latter, shedding light on the molecular mechanism of fusidic acid resistance. Nature Publishing Group 2016-01-19 /pmc/articles/PMC4725979/ /pubmed/26781961 http://dx.doi.org/10.1038/srep19524 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Tomlinson, Jennifer H.
Thompson, Gary S.
Kalverda, Arnout P.
Zhuravleva, Anastasia
O’Neill, Alex J.
A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title_full A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title_fullStr A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title_full_unstemmed A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title_short A target-protection mechanism of antibiotic resistance at atomic resolution: insights into FusB-type fusidic acid resistance
title_sort target-protection mechanism of antibiotic resistance at atomic resolution: insights into fusb-type fusidic acid resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4725979/
https://www.ncbi.nlm.nih.gov/pubmed/26781961
http://dx.doi.org/10.1038/srep19524
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