CRMAGE: CRISPR Optimized MAGE Recombineering

A bottleneck in metabolic engineering and systems biology approaches is the lack of efficient genome engineering technologies. Here, we combine CRISPR/Cas9 and λ Red recombineering based MAGE technology (CRMAGE) to create a highly efficient and fast method for genome engineering of Escherichia coli....

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Detalles Bibliográficos
Autores principales: Ronda, Carlotta, Pedersen, Lasse Ebdrup, Sommer, Morten O. A., Nielsen, Alex Toftgaard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726160/
https://www.ncbi.nlm.nih.gov/pubmed/26797514
http://dx.doi.org/10.1038/srep19452

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726160/
https://www.ncbi.nlm.nih.gov/pubmed/26797514
http://dx.doi.org/10.1038/srep19452

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