Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E. coli

The phytase gene appA(S) was isolated from Shigella sp. CD2 genomic library. The 3.8 kb DNA fragment contained 1299 bp open reading frame encoding 432 amino acid protein (AppA(S)) with 22 amino acid signal peptide at N-terminal and three sites of N-glycosylation. AppA(S) contained the active site RH...

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Detalles Bibliográficos
Autores principales: Pal Roy, Moushree, Mazumdar, Deepika, Dutta, Subhabrata, Saha, Shyama Prasad, Ghosh, Shilpi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726635/
https://www.ncbi.nlm.nih.gov/pubmed/26808559
http://dx.doi.org/10.1371/journal.pone.0145745

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726635/
https://www.ncbi.nlm.nih.gov/pubmed/26808559
http://dx.doi.org/10.1371/journal.pone.0145745

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