Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET

Despite recent advances, the structure and dynamics of membrane proteins in cell membranes remain elusive. We implemented transition metal ion fluorescence resonance energy transfer (tmFRET) to measure distances between sites on the N-terminal ankyrin repeat domains (ARDs) of the pain-transducing io...

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Detalles Bibliográficos
Autores principales: Zagotta, William N., Gordon, Moshe T., Senning, Eric N., Munari, Mika A., Gordon, Sharona E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2016
Materias:
Methods and Approaches
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727949/
https://www.ncbi.nlm.nih.gov/pubmed/26755770
http://dx.doi.org/10.1085/jgp.201511531

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727949/
https://www.ncbi.nlm.nih.gov/pubmed/26755770
http://dx.doi.org/10.1085/jgp.201511531

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