Cargando…

Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET

Despite recent advances, the structure and dynamics of membrane proteins in cell membranes remain elusive. We implemented transition metal ion fluorescence resonance energy transfer (tmFRET) to measure distances between sites on the N-terminal ankyrin repeat domains (ARDs) of the pain-transducing io...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zagotta, William N., Gordon, Moshe T., Senning, Eric N., Munari, Mika A., Gordon, Sharona E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 2016
Materias:	Methods and Approaches
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4727949/ https://www.ncbi.nlm.nih.gov/pubmed/26755770 http://dx.doi.org/10.1085/jgp.201511531

Ejemplares similares

Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane
por: Gordon, Sharona E., et al.
Publicado: (2016)

An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET
por: Zagotta, William N, et al.
Publicado: (2021)

Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands
por: Gordon, Sharona E., et al.
Publicado: (2023)

Activity and Ca(2+) regulate the mobility of TRPV1 channels in the plasma membrane of sensory neurons
por: Senning, Eric N, et al.
Publicado: (2015)

Visualizing conformational dynamics of proteins in solution and at the cell membrane
por: Gordon, Sharona E, et al.
Publicado: (2018)

Correction: Visualizing conformational dynamics of proteins in solution and at the cell membrane
por: Gordon, Sharona E, et al.
Publicado: (2018)

Ca(2+)/Calmodulin Modulates TRPV1 Activation by Capsaicin
por: Rosenbaum, Tamara, et al.
Publicado: (2004)

Mapping the structure and conformational movements of proteins with transition metal ion FRET
por: Taraska, Justin W., et al.
Publicado: (2009)

Reciprocal regulation among TRPV1 channels and phosphoinositide 3-kinase in response to nerve growth factor
por: Stratiievska, Anastasiia, et al.
Publicado: (2018)

Measuring conformational equilibria in allosteric proteins with time-resolved tmFRET
por: Zagotta, William N., et al.
Publicado: (2023)

Functional characterization of a ClC transporter by solid-supported membrane electrophysiology
por: Garcia-Celma, Juan, et al.
Publicado: (2013)

Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS
por: Manno, Carlo, et al.
Publicado: (2013)

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs
por: Shintani, Seine A., et al.
Publicado: (2014)

Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin
por: Xu, Ji, et al.
Publicado: (2014)

Calmodulation meta-analysis: Predicting calmodulin binding via canonical motif clustering
por: Mruk, Karen, et al.
Publicado: (2014)

Molecular dynamics simulations of membrane proteins under asymmetric ionic concentrations
por: Khalili-Araghi, Fatemeh, et al.
Publicado: (2013)

Determination of parameter identifiability in nonlinear biophysical models: A Bayesian approach
por: Hines, Keegan E., et al.
Publicado: (2014)

Inferring subunit stoichiometry from single molecule photobleaching
por: Hines, Keegan E.
Publicado: (2013)

Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na(+)-driven transporters
por: Ravera, Silvia, et al.
Publicado: (2015)

Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules
por: Tong, Xuhui, et al.
Publicado: (2015)

Zinc flexes its muscle: Correcting a novel analysis of calcium for zinc interference uncovers a method to measure zinc
por: Qian, Cheng, et al.
Publicado: (2016)

bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
por: Zheng, Hui, et al.
Publicado: (2016)

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
por: Lamboley, Cédric R.H., et al.
Publicado: (2015)

Stabilization of a prokaryotic LAT transporter by random mutagenesis
por: Rodríguez-Banqueri, Arturo, et al.
Publicado: (2016)

Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses
por: Shigetomi, Eiji, et al.
Publicado: (2013)

A novel voltage-clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels
por: Gaete, Pablo S., et al.
Publicado: (2020)

Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes
por: Zhang, Lejie, et al.
Publicado: (2020)

Action potentials in Xenopus oocytes triggered by blue light
por: Walther, Florian, et al.
Publicado: (2020)

A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin
por: Bunch, Thomas A., et al.
Publicado: (2021)

A method for imaging single molecules at the plasma membrane of live cells within tissue slices
por: Mashanov, Gregory I., et al.
Publicado: (2020)

A novel method for determining murine skeletal muscle fiber type using autofluorescence lifetimes
por: Manno, Carlo, et al.
Publicado: (2022)

A reconstituted depolarization-induced Ca(2+) release platform for validation of skeletal muscle disease mutations and drug discovery
por: Murayama, Takashi, et al.
Publicado: (2022)

Rationally evolving tRNA(Pyl) for efficient incorporation of noncanonical amino acids
por: Fan, Chenguang, et al.
Publicado: (2015)

Noncanonical amino acid mutagenesis in response to recoding signal-enhanced quadruplet codons
por: Chen, Yan, et al.
Publicado: (2022)

Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells
por: Jones, Chloe M., et al.
Publicado: (2021)

Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids
por: Jana, Subhashis, et al.
Publicado: (2023)

Mechanism for phosphoinositide selectivity and activation of TRPV1 ion channels
por: Ufret-Vincenty, Carmen A., et al.
Publicado: (2015)

Mechanism of Tetracaine Block of Cyclic Nucleotide-gated Channels
por: Fodor, Anthony A., et al.
Publicado: (1997)

Phosphoinositide 3-Kinase Binds to TRPV1 and Mediates NGF-stimulated TRPV1 Trafficking to the Plasma Membrane
por: Stein, Alexander T., et al.
Publicado: (2006)

OptoPI3K, genetic code expansion, and click chemistry reveal mechanisms underlying reciprocal regulation between TRPV1 and PI3K
por: Koh, Duk-Su, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_3ji3r19ffmi3sqip31kv8h80m9