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Cloning, Expression, and Purification of Histidine-Tagged Escherichia coli Dihydrodipicolinate Reductase

The enzyme dihydrodipicolinate reductase (DHDPR) is a component of the lysine biosynthetic pathway in bacteria and higher plants. DHDPR catalyzes the NAD(P)H dependent reduction of 2,3-dihydrodipicolinate to the cyclic imine L-2,3,4,5,-tetrahydropicolinic acid. The dapB gene that encodes dihydrodipi...

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Detalles Bibliográficos
Autores principales: Trigoso, Yvonne D., Evans, Russell C., Karsten, William E., Chooback, Lilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729673/
https://www.ncbi.nlm.nih.gov/pubmed/26815040
http://dx.doi.org/10.1371/journal.pone.0146525