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Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729692/ https://www.ncbi.nlm.nih.gov/pubmed/26551560 http://dx.doi.org/10.7554/eLife.09571 |
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author | Zylicz, Jan J Dietmann, Sabine Günesdogan, Ufuk Hackett, Jamie A Cougot, Delphine Lee, Caroline Surani, M Azim |
author_facet | Zylicz, Jan J Dietmann, Sabine Günesdogan, Ufuk Hackett, Jamie A Cougot, Delphine Lee, Caroline Surani, M Azim |
author_sort | Zylicz, Jan J |
collection | PubMed |
description | Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with redistribution of enhancer of zeste homolog 2 (EZH2)-dependent histone H3 lysine 27 trimethylation (H3K27me3). Loss of G9a or EZH2 results in upregulation of distinct gene sets involved in cell cycle regulation, germline development and embryogenesis. Notably, the H3K9me2 modification extends to active enhancer elements where it promotes developmentally-linked gene silencing and directly marks promoters and gene bodies. This epigenetic mechanism is important for priming gene regulatory networks for critical cell fate decisions in rapidly proliferating postimplantation epiblast cells. DOI: http://dx.doi.org/10.7554/eLife.09571.001 |
format | Online Article Text |
id | pubmed-4729692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47296922016-03-17 Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development Zylicz, Jan J Dietmann, Sabine Günesdogan, Ufuk Hackett, Jamie A Cougot, Delphine Lee, Caroline Surani, M Azim eLife Developmental Biology and Stem Cells Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with redistribution of enhancer of zeste homolog 2 (EZH2)-dependent histone H3 lysine 27 trimethylation (H3K27me3). Loss of G9a or EZH2 results in upregulation of distinct gene sets involved in cell cycle regulation, germline development and embryogenesis. Notably, the H3K9me2 modification extends to active enhancer elements where it promotes developmentally-linked gene silencing and directly marks promoters and gene bodies. This epigenetic mechanism is important for priming gene regulatory networks for critical cell fate decisions in rapidly proliferating postimplantation epiblast cells. DOI: http://dx.doi.org/10.7554/eLife.09571.001 eLife Sciences Publications, Ltd 2015-11-09 /pmc/articles/PMC4729692/ /pubmed/26551560 http://dx.doi.org/10.7554/eLife.09571 Text en © 2015, Zylicz et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Zylicz, Jan J Dietmann, Sabine Günesdogan, Ufuk Hackett, Jamie A Cougot, Delphine Lee, Caroline Surani, M Azim Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title | Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title_full | Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title_fullStr | Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title_full_unstemmed | Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title_short | Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development |
title_sort | chromatin dynamics and the role of g9a in gene regulation and enhancer silencing during early mouse development |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729692/ https://www.ncbi.nlm.nih.gov/pubmed/26551560 http://dx.doi.org/10.7554/eLife.09571 |
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