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Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development

Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, a...

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Autores principales: Zylicz, Jan J, Dietmann, Sabine, Günesdogan, Ufuk, Hackett, Jamie A, Cougot, Delphine, Lee, Caroline, Surani, M Azim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729692/
https://www.ncbi.nlm.nih.gov/pubmed/26551560
http://dx.doi.org/10.7554/eLife.09571
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author Zylicz, Jan J
Dietmann, Sabine
Günesdogan, Ufuk
Hackett, Jamie A
Cougot, Delphine
Lee, Caroline
Surani, M Azim
author_facet Zylicz, Jan J
Dietmann, Sabine
Günesdogan, Ufuk
Hackett, Jamie A
Cougot, Delphine
Lee, Caroline
Surani, M Azim
author_sort Zylicz, Jan J
collection PubMed
description Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with redistribution of enhancer of zeste homolog 2 (EZH2)-dependent histone H3 lysine 27 trimethylation (H3K27me3). Loss of G9a or EZH2 results in upregulation of distinct gene sets involved in cell cycle regulation, germline development and embryogenesis. Notably, the H3K9me2 modification extends to active enhancer elements where it promotes developmentally-linked gene silencing and directly marks promoters and gene bodies. This epigenetic mechanism is important for priming gene regulatory networks for critical cell fate decisions in rapidly proliferating postimplantation epiblast cells. DOI: http://dx.doi.org/10.7554/eLife.09571.001
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spelling pubmed-47296922016-03-17 Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development Zylicz, Jan J Dietmann, Sabine Günesdogan, Ufuk Hackett, Jamie A Cougot, Delphine Lee, Caroline Surani, M Azim eLife Developmental Biology and Stem Cells Early mouse development is accompanied by dynamic changes in chromatin modifications, including G9a-mediated histone H3 lysine 9 dimethylation (H3K9me2), which is essential for embryonic development. Here we show that genome-wide accumulation of H3K9me2 is crucial for postimplantation development, and coincides with redistribution of enhancer of zeste homolog 2 (EZH2)-dependent histone H3 lysine 27 trimethylation (H3K27me3). Loss of G9a or EZH2 results in upregulation of distinct gene sets involved in cell cycle regulation, germline development and embryogenesis. Notably, the H3K9me2 modification extends to active enhancer elements where it promotes developmentally-linked gene silencing and directly marks promoters and gene bodies. This epigenetic mechanism is important for priming gene regulatory networks for critical cell fate decisions in rapidly proliferating postimplantation epiblast cells. DOI: http://dx.doi.org/10.7554/eLife.09571.001 eLife Sciences Publications, Ltd 2015-11-09 /pmc/articles/PMC4729692/ /pubmed/26551560 http://dx.doi.org/10.7554/eLife.09571 Text en © 2015, Zylicz et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Zylicz, Jan J
Dietmann, Sabine
Günesdogan, Ufuk
Hackett, Jamie A
Cougot, Delphine
Lee, Caroline
Surani, M Azim
Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title_full Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title_fullStr Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title_full_unstemmed Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title_short Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development
title_sort chromatin dynamics and the role of g9a in gene regulation and enhancer silencing during early mouse development
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729692/
https://www.ncbi.nlm.nih.gov/pubmed/26551560
http://dx.doi.org/10.7554/eLife.09571
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