A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus

A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-1...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ruichao, Chiou, Jiachi, Chan, Edward Wai-Chi, Chen, Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729947/
https://www.ncbi.nlm.nih.gov/pubmed/26858713
http://dx.doi.org/10.3389/fmicb.2016.00044
_version_ 1782412327441661952
author Li, Ruichao
Chiou, Jiachi
Chan, Edward Wai-Chi
Chen, Sheng
author_facet Li, Ruichao
Chiou, Jiachi
Chan, Edward Wai-Chi
Chen, Sheng
author_sort Li, Ruichao
collection PubMed
description A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this bla(CARB-17)-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification.
format Online
Article
Text
id pubmed-4729947
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-47299472016-02-08 A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus Li, Ruichao Chiou, Jiachi Chan, Edward Wai-Chi Chen, Sheng Front Microbiol Microbiology A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this bla(CARB-17)-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification. Frontiers Media S.A. 2016-01-28 /pmc/articles/PMC4729947/ /pubmed/26858713 http://dx.doi.org/10.3389/fmicb.2016.00044 Text en Copyright © 2016 Li, Chiou, Chan and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Li, Ruichao
Chiou, Jiachi
Chan, Edward Wai-Chi
Chen, Sheng
A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title_full A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title_fullStr A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title_full_unstemmed A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title_short A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
title_sort novel pcr-based approach for accurate identification of vibrio parahaemolyticus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729947/
https://www.ncbi.nlm.nih.gov/pubmed/26858713
http://dx.doi.org/10.3389/fmicb.2016.00044
work_keys_str_mv AT liruichao anovelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chioujiachi anovelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chanedwardwaichi anovelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chensheng anovelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT liruichao novelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chioujiachi novelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chanedwardwaichi novelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus
AT chensheng novelpcrbasedapproachforaccurateidentificationofvibrioparahaemolyticus