A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus
A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729947/ https://www.ncbi.nlm.nih.gov/pubmed/26858713 http://dx.doi.org/10.3389/fmicb.2016.00044 |
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author | Li, Ruichao Chiou, Jiachi Chan, Edward Wai-Chi Chen, Sheng |
author_facet | Li, Ruichao Chiou, Jiachi Chan, Edward Wai-Chi Chen, Sheng |
author_sort | Li, Ruichao |
collection | PubMed |
description | A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this bla(CARB-17)-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification. |
format | Online Article Text |
id | pubmed-4729947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47299472016-02-08 A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus Li, Ruichao Chiou, Jiachi Chan, Edward Wai-Chi Chen, Sheng Front Microbiol Microbiology A PCR-based assay was developed for more accurate identification of Vibrio parahaemolyticus through targeting the bla(CARB-17) like element, an intrinsic β-lactamase gene that may also be regarded as a novel species-specific genetic marker of this organism. Homologous analysis showed that bla(CARB-17) like genes were more conservative than the tlh, toxR and atpA genes, the genetic markers commonly used as detection targets in identification of V. parahaemolyticus. Our data showed that this bla(CARB-17)-specific PCR-based detection approach consistently achieved 100% specificity, whereas PCR targeting the tlh and atpA genes occasionally produced false positive results. Furthermore, a positive result of this test is consistently associated with an intrinsic ampicillin resistance phenotype of the test organism, presumably conferred by the products of bla(CARB-17) like genes. We envision that combined analysis of the unique genetic and phenotypic characteristics conferred by bla(CARB-17) shall further enhance the detection specificity of this novel yet easy-to-use detection approach to a level superior to the conventional methods used in V. parahaemolyticus detection and identification. Frontiers Media S.A. 2016-01-28 /pmc/articles/PMC4729947/ /pubmed/26858713 http://dx.doi.org/10.3389/fmicb.2016.00044 Text en Copyright © 2016 Li, Chiou, Chan and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Li, Ruichao Chiou, Jiachi Chan, Edward Wai-Chi Chen, Sheng A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title | A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title_full | A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title_fullStr | A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title_full_unstemmed | A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title_short | A Novel PCR-Based Approach for Accurate Identification of Vibrio parahaemolyticus |
title_sort | novel pcr-based approach for accurate identification of vibrio parahaemolyticus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4729947/ https://www.ncbi.nlm.nih.gov/pubmed/26858713 http://dx.doi.org/10.3389/fmicb.2016.00044 |
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