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Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells

BACKGROUND: Craniomaxillofacial defects secondary to trauma, tumor resection, or congenital malformations are frequent unmet challenges, due to suboptimal alloplastic options and limited autologous tissues such as bone. Significant advances have been made in the application of adipose-derived stem/s...

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Autores principales: Strong, Amy L., Hunter, Ryan S., Jones, Robert B., Bowles, Annie C., Dutreil, Maria F., Gaupp, Dina, Hayes, Daniel J., Gimble, Jeffrey M., Levi, Benjamin, McNulty, Margaret A., Bunnell, Bruce A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730660/
https://www.ncbi.nlm.nih.gov/pubmed/26818763
http://dx.doi.org/10.1186/s12967-016-0776-1
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author Strong, Amy L.
Hunter, Ryan S.
Jones, Robert B.
Bowles, Annie C.
Dutreil, Maria F.
Gaupp, Dina
Hayes, Daniel J.
Gimble, Jeffrey M.
Levi, Benjamin
McNulty, Margaret A.
Bunnell, Bruce A.
author_facet Strong, Amy L.
Hunter, Ryan S.
Jones, Robert B.
Bowles, Annie C.
Dutreil, Maria F.
Gaupp, Dina
Hayes, Daniel J.
Gimble, Jeffrey M.
Levi, Benjamin
McNulty, Margaret A.
Bunnell, Bruce A.
author_sort Strong, Amy L.
collection PubMed
description BACKGROUND: Craniomaxillofacial defects secondary to trauma, tumor resection, or congenital malformations are frequent unmet challenges, due to suboptimal alloplastic options and limited autologous tissues such as bone. Significant advances have been made in the application of adipose-derived stem/stromal cells (ASCs) in the pre-clinical and clinical settings as a cell source for tissue engineering approaches. To fully realize the translational potential of ASCs, the identification of optimal donors for ASCs will ensure the successful implementation of these cells for tissue engineering approaches. In the current study, the impact of obesity on the osteogenic differentiation of ASCs was investigated. METHODS: ASCs isolated from lean donors (body mass index <25; lnASCs) and obese donors (body mass index >30; obASCs) were induced with osteogenic differentiation medium as monolayers in an estrogen-depleted culture system and on three-dimensional scaffolds. Critical size calvarial defects were generated in male nude mice and treated with scaffolds implanted with lnASCs or obASCs. RESULTS: lnASCs demonstrated enhanced osteogenic differentiation in monolayer culture system, on three-dimensional scaffolds, and for the treatment of calvarial defects, whereas obASCs were unable to induce similar levels of osteogenic differentiation in vitro and in vivo. Gene expression analysis of lnASCs and obASCs during osteogenic differentiation demonstrated higher levels of osteogenic genes in lnASCs compared to obASCs. CONCLUSION: Collectively, these results indicate that obesity reduces the osteogenic differentiation capacity of ASCs such that they may have a limited suitability as a cell source for tissue engineering. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12967-016-0776-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-47306602016-01-29 Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells Strong, Amy L. Hunter, Ryan S. Jones, Robert B. Bowles, Annie C. Dutreil, Maria F. Gaupp, Dina Hayes, Daniel J. Gimble, Jeffrey M. Levi, Benjamin McNulty, Margaret A. Bunnell, Bruce A. J Transl Med Research BACKGROUND: Craniomaxillofacial defects secondary to trauma, tumor resection, or congenital malformations are frequent unmet challenges, due to suboptimal alloplastic options and limited autologous tissues such as bone. Significant advances have been made in the application of adipose-derived stem/stromal cells (ASCs) in the pre-clinical and clinical settings as a cell source for tissue engineering approaches. To fully realize the translational potential of ASCs, the identification of optimal donors for ASCs will ensure the successful implementation of these cells for tissue engineering approaches. In the current study, the impact of obesity on the osteogenic differentiation of ASCs was investigated. METHODS: ASCs isolated from lean donors (body mass index <25; lnASCs) and obese donors (body mass index >30; obASCs) were induced with osteogenic differentiation medium as monolayers in an estrogen-depleted culture system and on three-dimensional scaffolds. Critical size calvarial defects were generated in male nude mice and treated with scaffolds implanted with lnASCs or obASCs. RESULTS: lnASCs demonstrated enhanced osteogenic differentiation in monolayer culture system, on three-dimensional scaffolds, and for the treatment of calvarial defects, whereas obASCs were unable to induce similar levels of osteogenic differentiation in vitro and in vivo. Gene expression analysis of lnASCs and obASCs during osteogenic differentiation demonstrated higher levels of osteogenic genes in lnASCs compared to obASCs. CONCLUSION: Collectively, these results indicate that obesity reduces the osteogenic differentiation capacity of ASCs such that they may have a limited suitability as a cell source for tissue engineering. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12967-016-0776-1) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-27 /pmc/articles/PMC4730660/ /pubmed/26818763 http://dx.doi.org/10.1186/s12967-016-0776-1 Text en © Strong et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Strong, Amy L.
Hunter, Ryan S.
Jones, Robert B.
Bowles, Annie C.
Dutreil, Maria F.
Gaupp, Dina
Hayes, Daniel J.
Gimble, Jeffrey M.
Levi, Benjamin
McNulty, Margaret A.
Bunnell, Bruce A.
Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title_full Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title_fullStr Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title_full_unstemmed Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title_short Obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
title_sort obesity inhibits the osteogenic differentiation of human adipose-derived stem cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4730660/
https://www.ncbi.nlm.nih.gov/pubmed/26818763
http://dx.doi.org/10.1186/s12967-016-0776-1
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