Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells

Valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA) are both HDAC inhibitors (HDACi). Previous studies indicated that both inhibitors show therapeutic effects on acute myeloid leukaemia (AML), while the differential impacts of the two different HDACi on AML treatment still remains elusive...

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Autores principales: Zhu, Xiaoyu, Liu, Xin, Cheng, Zhongyi, Zhu, Jun, Xu, Lei, Wang, Fengsong, Qi, Wulin, Yan, Jiawei, Liu, Ning, Sun, Zimin, Liu, Huilan, Peng, Xiaojun, Hao, Yingchan, Zheng, Nan, Wu, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731804/
https://www.ncbi.nlm.nih.gov/pubmed/26822725
http://dx.doi.org/10.1038/srep19926
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author Zhu, Xiaoyu
Liu, Xin
Cheng, Zhongyi
Zhu, Jun
Xu, Lei
Wang, Fengsong
Qi, Wulin
Yan, Jiawei
Liu, Ning
Sun, Zimin
Liu, Huilan
Peng, Xiaojun
Hao, Yingchan
Zheng, Nan
Wu, Quan
author_facet Zhu, Xiaoyu
Liu, Xin
Cheng, Zhongyi
Zhu, Jun
Xu, Lei
Wang, Fengsong
Qi, Wulin
Yan, Jiawei
Liu, Ning
Sun, Zimin
Liu, Huilan
Peng, Xiaojun
Hao, Yingchan
Zheng, Nan
Wu, Quan
author_sort Zhu, Xiaoyu
collection PubMed
description Valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA) are both HDAC inhibitors (HDACi). Previous studies indicated that both inhibitors show therapeutic effects on acute myeloid leukaemia (AML), while the differential impacts of the two different HDACi on AML treatment still remains elusive. In this study, using 3-plex SILAC based quantitative proteomics technique, anti-acetyllysine antibody based affinity enrichment, high resolution LC-MS/MS and intensive bioinformatic analysis, the quantitative proteome and acetylome in SAHA and VPA treated AML HL60 cells were extensively studied. In total, 5,775 proteins and 1,124 lysine acetylation sites were successfully obtained in response to VAP and SAHA treatment. It is found that VPA and SAHA treatment differently induced proteome and acetylome profiling in AML HL60 cells. This study revealed the differential impacts of VPA and SAHA on proteome/acetylome in AML cells, deepening our understanding of HDAC inhibitor mediated AML therapeutics.
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spelling pubmed-47318042016-02-04 Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells Zhu, Xiaoyu Liu, Xin Cheng, Zhongyi Zhu, Jun Xu, Lei Wang, Fengsong Qi, Wulin Yan, Jiawei Liu, Ning Sun, Zimin Liu, Huilan Peng, Xiaojun Hao, Yingchan Zheng, Nan Wu, Quan Sci Rep Article Valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA) are both HDAC inhibitors (HDACi). Previous studies indicated that both inhibitors show therapeutic effects on acute myeloid leukaemia (AML), while the differential impacts of the two different HDACi on AML treatment still remains elusive. In this study, using 3-plex SILAC based quantitative proteomics technique, anti-acetyllysine antibody based affinity enrichment, high resolution LC-MS/MS and intensive bioinformatic analysis, the quantitative proteome and acetylome in SAHA and VPA treated AML HL60 cells were extensively studied. In total, 5,775 proteins and 1,124 lysine acetylation sites were successfully obtained in response to VAP and SAHA treatment. It is found that VPA and SAHA treatment differently induced proteome and acetylome profiling in AML HL60 cells. This study revealed the differential impacts of VPA and SAHA on proteome/acetylome in AML cells, deepening our understanding of HDAC inhibitor mediated AML therapeutics. Nature Publishing Group 2016-01-29 /pmc/articles/PMC4731804/ /pubmed/26822725 http://dx.doi.org/10.1038/srep19926 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhu, Xiaoyu
Liu, Xin
Cheng, Zhongyi
Zhu, Jun
Xu, Lei
Wang, Fengsong
Qi, Wulin
Yan, Jiawei
Liu, Ning
Sun, Zimin
Liu, Huilan
Peng, Xiaojun
Hao, Yingchan
Zheng, Nan
Wu, Quan
Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title_full Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title_fullStr Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title_full_unstemmed Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title_short Quantitative Analysis of Global Proteome and Lysine Acetylome Reveal the Differential Impacts of VPA and SAHA on HL60 Cells
title_sort quantitative analysis of global proteome and lysine acetylome reveal the differential impacts of vpa and saha on hl60 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731804/
https://www.ncbi.nlm.nih.gov/pubmed/26822725
http://dx.doi.org/10.1038/srep19926
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