Cargando…

Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites

BACKGROUND: Malaria parasites have evolved a series of intricate mechanisms to survive and propagate within host red blood cells. Intra-erythrocytic parasitism requires these organisms to digest haemoglobin and detoxify iron-bound haem. These tasks are executed by haemoglobin-specific proteases and...

Descripción completa

Detalles Bibliográficos
Autores principales: Ponsuwanna, Patrath, Kochakarn, Theerarat, Bunditvorapoom, Duangkamon, Kümpornsin, Krittikorn, Otto, Thomas D., Ridenour, Chase, Chotivanich, Kesinee, Wilairat, Prapon, White, Nicholas J., Miotto, Olivo, Chookajorn, Thanat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731938/
https://www.ncbi.nlm.nih.gov/pubmed/26821618
http://dx.doi.org/10.1186/s12936-016-1097-9
_version_ 1782412619318034432
author Ponsuwanna, Patrath
Kochakarn, Theerarat
Bunditvorapoom, Duangkamon
Kümpornsin, Krittikorn
Otto, Thomas D.
Ridenour, Chase
Chotivanich, Kesinee
Wilairat, Prapon
White, Nicholas J.
Miotto, Olivo
Chookajorn, Thanat
author_facet Ponsuwanna, Patrath
Kochakarn, Theerarat
Bunditvorapoom, Duangkamon
Kümpornsin, Krittikorn
Otto, Thomas D.
Ridenour, Chase
Chotivanich, Kesinee
Wilairat, Prapon
White, Nicholas J.
Miotto, Olivo
Chookajorn, Thanat
author_sort Ponsuwanna, Patrath
collection PubMed
description BACKGROUND: Malaria parasites have evolved a series of intricate mechanisms to survive and propagate within host red blood cells. Intra-erythrocytic parasitism requires these organisms to digest haemoglobin and detoxify iron-bound haem. These tasks are executed by haemoglobin-specific proteases and haem biocrystallization factors that are components of a large multi-subunit complex. Since haemoglobin processing machineries are functionally and genetically linked to the modes of action and resistance mechanisms of several anti-malarial drugs, an understanding of their evolutionary history is important for drug development and drug resistance prevention. METHODS: Maximum likelihood trees of genetic repertoires encoding haemoglobin processing machineries within Plasmodium species, and with the representatives of Apicomplexan species with various host tropisms, were created. Genetic variants were mapped onto existing three-dimensional structures. Genome-wide single nucleotide polymorphism data were used to analyse the selective pressure and the effect of these mutations at the structural level. RESULTS: Recent expansions in the falcipain and plasmepsin repertoires are unique to human malaria parasites especially in the Plasmodium falciparum and P. reichenowi lineage. Expansion of haemoglobin-specific plasmepsins occurred after the separation event of Plasmodium species, but the other members of the plasmepsin family were evolutionarily conserved with one copy for each sub-group in every Apicomplexan species. Haemoglobin-specific falcipains are separated from invasion-related falcipain, and their expansions within one specific locus arose independently in both P. falciparum and P. vivax lineages. Gene conversion between P. falciparum falcipain 2A and 2B was observed in artemisinin-resistant strains. Comparison between the numbers of non-synonymous and synonymous mutations suggests a strong selective pressure at falcipain and plasmepsin genes. The locations of amino acid changes from non-synonymous mutations mapped onto protein structures revealed clusters of amino acid residues in close proximity or near the active sites of proteases. CONCLUSION: A high degree of polymorphism at the haemoglobin processing genes implicates an imposition of selective pressure. The identification in recent years of functional redundancy of haemoglobin-specific proteases makes them less appealing as potential drug targets, but their expansions, especially in the human malaria parasite lineages, unequivocally point toward their functional significance during the independent and repetitive adaptation events in malaria parasite evolutionary history. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-016-1097-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4731938
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-47319382016-01-30 Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites Ponsuwanna, Patrath Kochakarn, Theerarat Bunditvorapoom, Duangkamon Kümpornsin, Krittikorn Otto, Thomas D. Ridenour, Chase Chotivanich, Kesinee Wilairat, Prapon White, Nicholas J. Miotto, Olivo Chookajorn, Thanat Malar J Research BACKGROUND: Malaria parasites have evolved a series of intricate mechanisms to survive and propagate within host red blood cells. Intra-erythrocytic parasitism requires these organisms to digest haemoglobin and detoxify iron-bound haem. These tasks are executed by haemoglobin-specific proteases and haem biocrystallization factors that are components of a large multi-subunit complex. Since haemoglobin processing machineries are functionally and genetically linked to the modes of action and resistance mechanisms of several anti-malarial drugs, an understanding of their evolutionary history is important for drug development and drug resistance prevention. METHODS: Maximum likelihood trees of genetic repertoires encoding haemoglobin processing machineries within Plasmodium species, and with the representatives of Apicomplexan species with various host tropisms, were created. Genetic variants were mapped onto existing three-dimensional structures. Genome-wide single nucleotide polymorphism data were used to analyse the selective pressure and the effect of these mutations at the structural level. RESULTS: Recent expansions in the falcipain and plasmepsin repertoires are unique to human malaria parasites especially in the Plasmodium falciparum and P. reichenowi lineage. Expansion of haemoglobin-specific plasmepsins occurred after the separation event of Plasmodium species, but the other members of the plasmepsin family were evolutionarily conserved with one copy for each sub-group in every Apicomplexan species. Haemoglobin-specific falcipains are separated from invasion-related falcipain, and their expansions within one specific locus arose independently in both P. falciparum and P. vivax lineages. Gene conversion between P. falciparum falcipain 2A and 2B was observed in artemisinin-resistant strains. Comparison between the numbers of non-synonymous and synonymous mutations suggests a strong selective pressure at falcipain and plasmepsin genes. The locations of amino acid changes from non-synonymous mutations mapped onto protein structures revealed clusters of amino acid residues in close proximity or near the active sites of proteases. CONCLUSION: A high degree of polymorphism at the haemoglobin processing genes implicates an imposition of selective pressure. The identification in recent years of functional redundancy of haemoglobin-specific proteases makes them less appealing as potential drug targets, but their expansions, especially in the human malaria parasite lineages, unequivocally point toward their functional significance during the independent and repetitive adaptation events in malaria parasite evolutionary history. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12936-016-1097-9) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-29 /pmc/articles/PMC4731938/ /pubmed/26821618 http://dx.doi.org/10.1186/s12936-016-1097-9 Text en © Ponsuwanna et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ponsuwanna, Patrath
Kochakarn, Theerarat
Bunditvorapoom, Duangkamon
Kümpornsin, Krittikorn
Otto, Thomas D.
Ridenour, Chase
Chotivanich, Kesinee
Wilairat, Prapon
White, Nicholas J.
Miotto, Olivo
Chookajorn, Thanat
Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title_full Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title_fullStr Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title_full_unstemmed Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title_short Comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
title_sort comparative genome-wide analysis and evolutionary history of haemoglobin-processing and haem detoxification enzymes in malarial parasites
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4731938/
https://www.ncbi.nlm.nih.gov/pubmed/26821618
http://dx.doi.org/10.1186/s12936-016-1097-9
work_keys_str_mv AT ponsuwannapatrath comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT kochakarntheerarat comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT bunditvorapoomduangkamon comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT kumpornsinkrittikorn comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT ottothomasd comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT ridenourchase comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT chotivanichkesinee comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT wilairatprapon comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT whitenicholasj comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT miottoolivo comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites
AT chookajornthanat comparativegenomewideanalysisandevolutionaryhistoryofhaemoglobinprocessingandhaemdetoxificationenzymesinmalarialparasites