Effects of CreER(T2), 4-OH Tamoxifen, and Gender on CFU-F Assays

Gene function in stem cell maintenance is often tested by inducing deletion via the Cre-loxP system. However, controls for Cre and other variables are frequently not included. Here we show that when cultured in the presence of 4-OH tamoxifen, bone and marrow cells containing the CreER(T2) construct...

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Detalles Bibliográficos
Autores principales: McHaffie, Sophie L., Hastie, Nicholas D., Chau, You-Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734617/
https://www.ncbi.nlm.nih.gov/pubmed/26828722
http://dx.doi.org/10.1371/journal.pone.0148105

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734617/
https://www.ncbi.nlm.nih.gov/pubmed/26828722
http://dx.doi.org/10.1371/journal.pone.0148105

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