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Effects of CreER(T2), 4-OH Tamoxifen, and Gender on CFU-F Assays
Gene function in stem cell maintenance is often tested by inducing deletion via the Cre-loxP system. However, controls for Cre and other variables are frequently not included. Here we show that when cultured in the presence of 4-OH tamoxifen, bone and marrow cells containing the CreER(T2) construct...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734617/ https://www.ncbi.nlm.nih.gov/pubmed/26828722 http://dx.doi.org/10.1371/journal.pone.0148105 |