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Development of an Analysis Pipeline Characterizing Multiple Hypervariable Regions of 16S rRNA Using Mock Samples

OBJECTIVES: There is much speculation on which hypervariable region provides the highest bacterial specificity in 16S rRNA sequencing. The optimum solution to prevent bias and to obtain a comprehensive view of complex bacterial communities would be to sequence the entire 16S rRNA gene; however, this...

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Detalles Bibliográficos
Autores principales: Barb, Jennifer J., Oler, Andrew J., Kim, Hyung-Suk, Chalmers, Natalia, Wallen, Gwenyth R., Cashion, Ann, Munson, Peter J., Ames, Nancy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4734828/
https://www.ncbi.nlm.nih.gov/pubmed/26829716
http://dx.doi.org/10.1371/journal.pone.0148047