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Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction

Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing...

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Detalles Bibliográficos
Autores principales: Vuong, Jeni, Collard, Jean-Marc, Whaley, Melissa J., Bassira, Issaka, Seidou, Issaka, Diarra, Seydou, Ouédraogo, Rasmata T., Kambiré, Dinanibè, Taylor, Thomas H., Sacchi, Claudio, Mayer, Leonard W., Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735509/
https://www.ncbi.nlm.nih.gov/pubmed/26829233
http://dx.doi.org/10.1371/journal.pone.0147765