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Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction
Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing...
Autores principales: | Vuong, Jeni, Collard, Jean-Marc, Whaley, Melissa J., Bassira, Issaka, Seidou, Issaka, Diarra, Seydou, Ouédraogo, Rasmata T., Kambiré, Dinanibè, Taylor, Thomas H., Sacchi, Claudio, Mayer, Leonard W., Wang, Xin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735509/ https://www.ncbi.nlm.nih.gov/pubmed/26829233 http://dx.doi.org/10.1371/journal.pone.0147765 |
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