Cargando…

Luciferase mRNA Transfection of Antigen Presenting Cells Permits Sensitive Nonradioactive Measurement of Cellular and Humoral Cytotoxicity

Immunotherapy is rapidly evolving as an effective treatment option for many cancers. With the emerging fields of cancer vaccines and adoptive cell transfer therapies, there is an increasing demand for high-throughput in vitro cytotoxicity assays that efficiently analyze immune effector functions. Th...

Descripción completa

Detalles Bibliográficos
Autores principales:	Omokoko, Tana A., Luxemburger, Uli, Bardissi, Shaheer, Simon, Petra, Utsch, Magdalena, Breitkreuz, Andrea, Türeci, Özlem, Sahin, Ugur
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736447/ https://www.ncbi.nlm.nih.gov/pubmed/27057556 http://dx.doi.org/10.1155/2016/9540975

Ejemplares similares

Retrieval of functional TCRs from single antigen-specific T cells: Toward individualized TCR-engineered therapies
por: Omokoko, Tana, et al.
Publicado: (2015)

CXorf61 is a target for T cell based immunotherapy of triple-negative breast cancer
por: Paret, Claudia, et al.
Publicado: (2015)

Methods in nonradioactive detection
Publicado: (1993)

Racing for a SARS‐CoV‐2 vaccine
por: Türeci, Özlem, et al.
Publicado: (2021)

Applications of stable, nonradioactive isotope tracers in in vivo human metabolic research
por: Kim, Il-Young, et al.
Publicado: (2016)

Nonradioactive direct telomerase activity detection using biotin‐labeled primers
por: Wang, Ruiguan, et al.
Publicado: (2021)

The Influence of Hypoxia and pH on Bioluminescence Imaging of Luciferase-Transfected Tumor Cells and Xenografts
por: Khalil, Ashraf A., et al.
Publicado: (2013)

Selective activation of tumor growth-promoting Ca(2+ )channel MS4A12 in colon cancer by caudal type homeobox transcription factor CDX2
por: Koslowski, Michael, et al.
Publicado: (2009)

Biocompatibility, Inflammatory Response, and Recannalization Characteristics of Nonradioactive Resin Microspheres: Histological Findings
por: Bilbao, José I., et al.
Publicado: (2009)

The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cells
por: Jiwaji, Meesbah, et al.
Publicado: (2010)

Targeting the tumor mutanome for personalized vaccination therapy
por: Kreiter, Sebastian, et al.
Publicado: (2012)

Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the human malaria parasite Plasmodium falciparum
por: Merrick, Catherine J.
Publicado: (2015)

International Symposium on the Feasibility of Aneutronic Power : Review of Searches for Nonradioactive Nonproliferating Nuclear Energy
por: Maglic, Bogdan C, et al.
Publicado: (1988)

Retracted: Luciferase-transfected colon adenocarcinoma cell line (DLD-1) for use in Orthotopic Xenotransplantation studies
por: Siddique, Muhammad Rashid, et al.
Publicado: (2012)

Retraction: Luciferase-transfected colon adenocarcinoma cell line (DLD-1) for use in Orthotopic Xenotransplantation studies
por: Siddique, Muhammad Rashid, et al.
Publicado: (2012)

Nonradioactive heteroduplex tracking assay for the detection of minority-variant chloroquine-resistant Plasmodium falciparum in Madagascar
por: Juliano, Jonathan J, et al.
Publicado: (2009)

Distinguishing Persistent Insulin Autoantibodies With Differential Risk: Nonradioactive Bivalent Proinsulin/Insulin Autoantibody Assay
por: Yu, Liping, et al.
Publicado: (2012)

2nd International Symposium on Aneutronic Power : Review of Searches for Nonradioactive Nonproliferating Nuclear Energy
Publicado: (1989)

Higher Sensitivity and Earlier Identification of Celiac Disease Autoimmunity by a Nonradioactive Assay for Transglutaminase Autoantibodies
por: Zhao, Zhiyuan, et al.
Publicado: (2016)

Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
por: Wu, Wei, et al.
Publicado: (2014)

Development and characterization of a novel luciferase based cytotoxicity assay
por: Matta, Hittu, et al.
Publicado: (2018)

A novel thermostable beetle luciferase based cytotoxicity assay
por: Choi, Sunju, et al.
Publicado: (2021)

A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C(6)-Ceramide
por: Van Overloop, Helena, et al.
Publicado: (2012)

Glucose Metabolic Trapping in Mouse Arteries: Nonradioactive Assay of Atherosclerotic Plaque Inflammation Applicable to Drug Discovery
por: Conway, Richard G., et al.
Publicado: (2012)

A simple nonradioactive method for the determination of the binding affinities of antibodies induced by hapten bioconjugates for drugs of abuse
por: Torres, Oscar B., et al.
Publicado: (2015)

A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors
por: Henrich, Curtis J.
Publicado: (2016)

Molecular Characterization of Virus-induced Autoantibody Responses
por: Ludewig, Burkhard, et al.
Publicado: (2004)

NCOA3 is a selective co-activator of estrogen receptor α-mediated transactivation of PLAC1 in MCF-7 breast cancer cells
por: Wagner, Meike, et al.
Publicado: (2013)

Mutanome Engineered RNA Immunotherapy: Towards Patient-Centered Tumor Vaccination
por: Vormehr, Mathias, et al.
Publicado: (2015)

Comparison of Claudin 18.2 expression in primary tumors and lymph node metastases in Japanese patients with gastric adenocarcinoma
por: Rohde, Christoph, et al.
Publicado: (2019)

Immunological effects and activity of multiple doses of zolbetuximab in combination with zoledronic acid and interleukin-2 in a phase 1 study in patients with advanced gastric and gastroesophageal junction cancer
por: Lordick, Florian, et al.
Publicado: (2023)

Assessment of Nonradioactive Multispectral Optoacoustic Tomographic Imaging With Conventional Lymphoscintigraphic Imaging for Sentinel Lymph Node Biopsy in Melanoma
por: Stoffels, Ingo, et al.
Publicado: (2019)

Tolerance of High Oral Doses of Nonradioactive and Radioactive Caesium Chloride in the Pale Grass Blue Butterfly Zizeeria maha
por: Gurung, Raj D., et al.
Publicado: (2019)

Patient-reported outcomes from the phase II FAST trial of zolbetuximab plus EOX compared to EOX alone as first-line treatment of patients with metastatic CLDN18.2+ gastroesophageal adenocarcinoma
por: Lordick, Florian, et al.
Publicado: (2021)

Evaluation of the nonradioactive inducible magnetic seed system Magseed for preoperative localization of nonpalpable breast lesions – initial clinical experience
por: Pieszko, Karolina, et al.
Publicado: (2020)

Plasmodium falciparum Transfected with Ultra Bright NanoLuc Luciferase Offers High Sensitivity Detection for the Screening of Growth and Cellular Trafficking Inhibitors
por: Azevedo, Mauro F., et al.
Publicado: (2014)

A Cytoplasmic Form of Gaussia luciferase Provides a Highly Sensitive Test for Cytotoxicity
por: Tsuji, Saori, et al.
Publicado: (2016)

Fast Sensing of Hydrogen Cyanide (HCN) Vapors Using a Hand-Held Ion Mobility Spectrometer with Nonradioactive Ionization Source
por: Bocos-Bintintan, Victor, et al.
Publicado: (2021)

The effect of cell penetrating peptides on transfection activity and cytotoxicity of polyallylamine
por: Sabouri-Rad, Sarvenaz, et al.
Publicado: (2017)

Reduced Cytotoxicity by Repetitive mRNA Transfection in Differentiated Neurons
por: Ko, Seung Hwan, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ise5q84o7qa5rmkgtn2e10po16