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In vitro directed evolution of alpha-hemolysin by liposome display

We have developed a method to enable in vitro directed evolution that can be applied to membrane proteins. This method, termed liposome display, uses liposomes as compartments in which membrane proteins are synthesized and as scaffolds for membrane protein integration. Thus, the synthesized membrane...

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Detalles Bibliográficos
Autores principales: Fujii, Satoshi, Matsuura, Tomoaki, Yomo, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736788/
https://www.ncbi.nlm.nih.gov/pubmed/27493517
http://dx.doi.org/10.2142/biophysics.11.67