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A protocol for preparing nucleotide-free KaiC monomer

The hexameric form of the KaiC protein is a core of the cyanobacterial biological clock, and its enzymatic activities exhibit circadian periodicity. The instability of the monomeric form of nucleotide-free KaiC has precluded its storage and detailed analyses of the activities of the reassembled hexa...

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Detalles Bibliográficos
Autores principales: Mukaiyama, Atsushi, Osako, Masato, Hikima, Takaaki, Kondo, Takao, Akiyama, Shuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan (BSJ) 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736791/
https://www.ncbi.nlm.nih.gov/pubmed/27493519
http://dx.doi.org/10.2142/biophysics.11.79