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A protocol for preparing nucleotide-free KaiC monomer
The hexameric form of the KaiC protein is a core of the cyanobacterial biological clock, and its enzymatic activities exhibit circadian periodicity. The instability of the monomeric form of nucleotide-free KaiC has precluded its storage and detailed analyses of the activities of the reassembled hexa...
Autores principales: | Mukaiyama, Atsushi, Osako, Masato, Hikima, Takaaki, Kondo, Takao, Akiyama, Shuji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Biophysical Society of Japan (BSJ)
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4736791/ https://www.ncbi.nlm.nih.gov/pubmed/27493519 http://dx.doi.org/10.2142/biophysics.11.79 |
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