Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP

In this paper, we asked if it is possible to identify the best primers and reaction conditions based on improvements in reaction speed when optimizing isothermal reactions. We used digital single-molecule, real-time analyses of both speed and efficiency of isothermal amplification reactions, which r...

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Detalles Bibliográficos
Autores principales: Khorosheva, Eugenia M., Karymov, Mikhail A., Selck, David A., Ismagilov, Rustem F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737171/
https://www.ncbi.nlm.nih.gov/pubmed/26358811
http://dx.doi.org/10.1093/nar/gkv877

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737171/
https://www.ncbi.nlm.nih.gov/pubmed/26358811
http://dx.doi.org/10.1093/nar/gkv877

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