CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis

We have developed a new, sequence-specific DNA labeling strategy that will dramatically improve DNA mapping in complex and structurally variant genomic regions, as well as facilitate high-throughput automated whole-genome mapping. The method uses the Cas9 D10A protein, which contains a nuclease disa...

Descripción completa

Detalles Bibliográficos
Autores principales: McCaffrey, Jennifer, Sibert, Justin, Zhang, Bin, Zhang, Yonggang, Hu, Wenhui, Riethman, Harold, Xiao, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737172/
https://www.ncbi.nlm.nih.gov/pubmed/26481349
http://dx.doi.org/10.1093/nar/gkv878

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737172/
https://www.ncbi.nlm.nih.gov/pubmed/26481349
http://dx.doi.org/10.1093/nar/gkv878

Ejemplares similares