IMAC capture of recombinant protein from unclarified mammalian cell feed streams

Fusion‐tag affinity chromatography is a key technique in recombinant protein purification. Current methods for protein recovery from mammalian cells are hampered by the need for feed stream clarification. We have developed a method for direct capture using immobilized metal affinity chromatography (...

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Detalles Bibliográficos
Autores principales: Kinna, Alexander, Tolner, Berend, Rota, Enrique Miranda, Titchener‐Hooker, Nigel, Nesbeth, Darren, Chester, Kerry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2015
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737217/
https://www.ncbi.nlm.nih.gov/pubmed/26174988
http://dx.doi.org/10.1002/bit.25705

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4737217/
https://www.ncbi.nlm.nih.gov/pubmed/26174988
http://dx.doi.org/10.1002/bit.25705

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