Cargando…

Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet

BACKGROUND: Emerging evidence suggested phytosterol esters (PE) exhibited an advantage over naturally occurring phytosterols in reducing atherosclerosis risk factors due to improved fat solubility and compatibility. However, the effects of dietary patterns of PE on lipid-lowering activity were limit...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Qianchun, Yu, Xiao, Xu, Jiqu, Kou, Xiuying, Zheng, Mingming, Huang, Fenghong, Huang, Qingde, Wang, Lan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739386/
https://www.ncbi.nlm.nih.gov/pubmed/26843021
http://dx.doi.org/10.1186/s12944-016-0185-8
_version_ 1782413738471587840
author Deng, Qianchun
Yu, Xiao
Xu, Jiqu
Kou, Xiuying
Zheng, Mingming
Huang, Fenghong
Huang, Qingde
Wang, Lan
author_facet Deng, Qianchun
Yu, Xiao
Xu, Jiqu
Kou, Xiuying
Zheng, Mingming
Huang, Fenghong
Huang, Qingde
Wang, Lan
author_sort Deng, Qianchun
collection PubMed
description BACKGROUND: Emerging evidence suggested phytosterol esters (PE) exhibited an advantage over naturally occurring phytosterols in reducing atherosclerosis risk factors due to improved fat solubility and compatibility. However, the effects of dietary patterns of PE on lipid-lowering activity were limited and inconsistent. This study aimed to explore the effects of dose and frequency of α-linolenic acid rich phytosterol esters (ALA-PE) on cholesterol and triglyceride metabolism markers focused on intestinal cholesterol absorption and bioconversion of ALA in liver. METHODS: Dose-dependency study Male Syrian golden hamsters were fed high-fat diets (HFD) containing low, medium and high dose of ALA-PE (0.72 %, 2.13 % and 6.39 %) for 6 weeks. The high fat diet contained 89.5 % chow diet, 0.2 % cholesterol, 10 % lard and 0.3 % bile salt. Dose-frequency study Male Syrian golden hamsters were provided: (I) 0.4 mL/100 g peanut oil by gavage once a day; (II) 0.4 mL/100 g ALA-PE by gavage once a day; (III) 0.2 mL/100 g ALA-PE by gavage twice a day; (IV) 0.133 mL/100 g ALA-PE by gavage three times a day; (V) 0.1 mL/100 g ALA-PE by gavage four times a day for 6 weeks with a high-fat diet simultaneously. RESULTS: ALA-PE dose-dependently lowered plasma total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) concentrations with a maximal decrease of 42 %, 59 % and 73 %, respectively (p < 0.05). Compared to HFD, TC, LDL-C and TG concentrations were significantly lower (p < 0.01) in hamsters consumed HFD plus ALA-PE for 1–4 times per day but there were not remarkable differences among different consumption frequencies. No significant changes in plasma antioxidant capacity and lipid peroxidation levels were observed among HFD and HFD plus different doses of ALA-PE groups. The contents of hepatic α-linolenic (ALA), docosapentaenoic (DPA) and docosahexaenoic (DHA) acids were dose-dependently increased in different ALA-PE groups compared to those in HFD group. The abundance of mRNA for intestinal sterol transporters Niemann-Pick C1-Like 1 (NPC1L1), ATP-binding cassette (ABC) transporters ABCG5 and ABCG8 indicated no significant differences among all groups. CONCLUSION: ALA-PE dose-dependently improved lipid profile in hamsters fed HFD independent of intestinal ABCG5, ABCG8 and NPC1L1, accompanying by increased conversion of ALA to DPA and DHA in liver. ALA-PE manifested “once a day” lipid-lowering efficacy, highlighting a promising preventive strategy for metabolic syndrome.
format Online
Article
Text
id pubmed-4739386
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-47393862016-02-04 Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet Deng, Qianchun Yu, Xiao Xu, Jiqu Kou, Xiuying Zheng, Mingming Huang, Fenghong Huang, Qingde Wang, Lan Lipids Health Dis Research BACKGROUND: Emerging evidence suggested phytosterol esters (PE) exhibited an advantage over naturally occurring phytosterols in reducing atherosclerosis risk factors due to improved fat solubility and compatibility. However, the effects of dietary patterns of PE on lipid-lowering activity were limited and inconsistent. This study aimed to explore the effects of dose and frequency of α-linolenic acid rich phytosterol esters (ALA-PE) on cholesterol and triglyceride metabolism markers focused on intestinal cholesterol absorption and bioconversion of ALA in liver. METHODS: Dose-dependency study Male Syrian golden hamsters were fed high-fat diets (HFD) containing low, medium and high dose of ALA-PE (0.72 %, 2.13 % and 6.39 %) for 6 weeks. The high fat diet contained 89.5 % chow diet, 0.2 % cholesterol, 10 % lard and 0.3 % bile salt. Dose-frequency study Male Syrian golden hamsters were provided: (I) 0.4 mL/100 g peanut oil by gavage once a day; (II) 0.4 mL/100 g ALA-PE by gavage once a day; (III) 0.2 mL/100 g ALA-PE by gavage twice a day; (IV) 0.133 mL/100 g ALA-PE by gavage three times a day; (V) 0.1 mL/100 g ALA-PE by gavage four times a day for 6 weeks with a high-fat diet simultaneously. RESULTS: ALA-PE dose-dependently lowered plasma total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) concentrations with a maximal decrease of 42 %, 59 % and 73 %, respectively (p < 0.05). Compared to HFD, TC, LDL-C and TG concentrations were significantly lower (p < 0.01) in hamsters consumed HFD plus ALA-PE for 1–4 times per day but there were not remarkable differences among different consumption frequencies. No significant changes in plasma antioxidant capacity and lipid peroxidation levels were observed among HFD and HFD plus different doses of ALA-PE groups. The contents of hepatic α-linolenic (ALA), docosapentaenoic (DPA) and docosahexaenoic (DHA) acids were dose-dependently increased in different ALA-PE groups compared to those in HFD group. The abundance of mRNA for intestinal sterol transporters Niemann-Pick C1-Like 1 (NPC1L1), ATP-binding cassette (ABC) transporters ABCG5 and ABCG8 indicated no significant differences among all groups. CONCLUSION: ALA-PE dose-dependently improved lipid profile in hamsters fed HFD independent of intestinal ABCG5, ABCG8 and NPC1L1, accompanying by increased conversion of ALA to DPA and DHA in liver. ALA-PE manifested “once a day” lipid-lowering efficacy, highlighting a promising preventive strategy for metabolic syndrome. BioMed Central 2016-02-03 /pmc/articles/PMC4739386/ /pubmed/26843021 http://dx.doi.org/10.1186/s12944-016-0185-8 Text en © Deng et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Deng, Qianchun
Yu, Xiao
Xu, Jiqu
Kou, Xiuying
Zheng, Mingming
Huang, Fenghong
Huang, Qingde
Wang, Lan
Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title_full Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title_fullStr Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title_full_unstemmed Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title_short Single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
title_sort single frequency intake of α-linolenic acid rich phytosterol esters attenuates atherosclerosis risk factors in hamsters fed a high fat diet
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739386/
https://www.ncbi.nlm.nih.gov/pubmed/26843021
http://dx.doi.org/10.1186/s12944-016-0185-8
work_keys_str_mv AT dengqianchun singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT yuxiao singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT xujiqu singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT kouxiuying singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT zhengmingming singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT huangfenghong singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT huangqingde singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet
AT wanglan singlefrequencyintakeofalinolenicacidrichphytosterolestersattenuatesatherosclerosisriskfactorsinhamstersfedahighfatdiet