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Protein stability: a crystallographer’s perspective

Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation...

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Detalles Bibliográficos
Autores principales: Deller, Marc C., Kong, Leopold, Rupp, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/
https://www.ncbi.nlm.nih.gov/pubmed/26841758
http://dx.doi.org/10.1107/S2053230X15024619
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author Deller, Marc C.
Kong, Leopold
Rupp, Bernhard
author_facet Deller, Marc C.
Kong, Leopold
Rupp, Bernhard
author_sort Deller, Marc C.
collection PubMed
description Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed.
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spelling pubmed-47411882016-02-13 Protein stability: a crystallographer’s perspective Deller, Marc C. Kong, Leopold Rupp, Bernhard Acta Crystallogr F Struct Biol Commun IYCr crystallization series Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed. International Union of Crystallography 2016-01-26 /pmc/articles/PMC4741188/ /pubmed/26841758 http://dx.doi.org/10.1107/S2053230X15024619 Text en © Deller et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle IYCr crystallization series
Deller, Marc C.
Kong, Leopold
Rupp, Bernhard
Protein stability: a crystallographer’s perspective
title Protein stability: a crystallographer’s perspective
title_full Protein stability: a crystallographer’s perspective
title_fullStr Protein stability: a crystallographer’s perspective
title_full_unstemmed Protein stability: a crystallographer’s perspective
title_short Protein stability: a crystallographer’s perspective
title_sort protein stability: a crystallographer’s perspective
topic IYCr crystallization series
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/
https://www.ncbi.nlm.nih.gov/pubmed/26841758
http://dx.doi.org/10.1107/S2053230X15024619
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