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Protein stability: a crystallographer’s perspective
Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/ https://www.ncbi.nlm.nih.gov/pubmed/26841758 http://dx.doi.org/10.1107/S2053230X15024619 |
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author | Deller, Marc C. Kong, Leopold Rupp, Bernhard |
author_facet | Deller, Marc C. Kong, Leopold Rupp, Bernhard |
author_sort | Deller, Marc C. |
collection | PubMed |
description | Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed. |
format | Online Article Text |
id | pubmed-4741188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-47411882016-02-13 Protein stability: a crystallographer’s perspective Deller, Marc C. Kong, Leopold Rupp, Bernhard Acta Crystallogr F Struct Biol Commun IYCr crystallization series Protein stability is a topic of major interest for the biotechnology, pharmaceutical and food industries, in addition to being a daily consideration for academic researchers studying proteins. An understanding of protein stability is essential for optimizing the expression, purification, formulation, storage and structural studies of proteins. In this review, discussion will focus on factors affecting protein stability, on a somewhat practical level, particularly from the view of a protein crystallographer. The differences between protein conformational stability and protein compositional stability will be discussed, along with a brief introduction to key methods useful for analyzing protein stability. Finally, tactics for addressing protein-stability issues during protein expression, purification and crystallization will be discussed. International Union of Crystallography 2016-01-26 /pmc/articles/PMC4741188/ /pubmed/26841758 http://dx.doi.org/10.1107/S2053230X15024619 Text en © Deller et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | IYCr crystallization series Deller, Marc C. Kong, Leopold Rupp, Bernhard Protein stability: a crystallographer’s perspective |
title | Protein stability: a crystallographer’s perspective |
title_full | Protein stability: a crystallographer’s perspective |
title_fullStr | Protein stability: a crystallographer’s perspective |
title_full_unstemmed | Protein stability: a crystallographer’s perspective |
title_short | Protein stability: a crystallographer’s perspective |
title_sort | protein stability: a crystallographer’s perspective |
topic | IYCr crystallization series |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741188/ https://www.ncbi.nlm.nih.gov/pubmed/26841758 http://dx.doi.org/10.1107/S2053230X15024619 |
work_keys_str_mv | AT dellermarcc proteinstabilityacrystallographersperspective AT kongleopold proteinstabilityacrystallographersperspective AT ruppbernhard proteinstabilityacrystallographersperspective |