A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour

Trans-kingdom conjugation is a phenomenon by which DNA is transferred into a eukaryotic cell by a bacterial conjugal transfer system. Improvement in this method to facilitate the rapid co-cultivation of donor bacterial and recipient eukaryotic cell cultures could make it the simplest transformation...

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Detalles Bibliográficos
Autores principales: Moriguchi, Kazuki, Yamamoto, Shinji, Ohmine, Yuta, Suzuki, Katsunori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744038/
https://www.ncbi.nlm.nih.gov/pubmed/26849654
http://dx.doi.org/10.1371/journal.pone.0148989

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744038/
https://www.ncbi.nlm.nih.gov/pubmed/26849654
http://dx.doi.org/10.1371/journal.pone.0148989

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