A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs

The genomes of more than 50 organisms have now been manipulated due to rapid advancement of gene editing technology. One way to perform gene editing in the mouse using the CRISPR/CAS system, guide RNA (gRNA) and CAS9 mRNA transcribed in vitro are microinjected into fertilized eggs that are then allo...

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Detalles Bibliográficos
Autores principales: Romanienko, Peter J., Giacalone, Joseph, Ingenito, Joanne, Wang, Yijie, Isaka, Mayumi, Johnson, Thomas, You, Yun, Mark, Willie H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744079/
https://www.ncbi.nlm.nih.gov/pubmed/26849369
http://dx.doi.org/10.1371/journal.pone.0148362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744079/
https://www.ncbi.nlm.nih.gov/pubmed/26849369
http://dx.doi.org/10.1371/journal.pone.0148362

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