Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), Cas9 can be reprogrammed to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary...

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Detalles Bibliográficos
Autores principales: Doench, John G., Fusi, Nicolo, Sullender, Meagan, Hegde, Mudra, Vaimberg, Emma W., Donovan, Katherine F., Smith, Ian, Tothova, Zuzana, Wilen, Craig, Orchard, Robert, Virgin, Herbert W., Listgarten, Jennifer, Root, David E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744125/
https://www.ncbi.nlm.nih.gov/pubmed/26780180
http://dx.doi.org/10.1038/nbt.3437

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744125/
https://www.ncbi.nlm.nih.gov/pubmed/26780180
http://dx.doi.org/10.1038/nbt.3437

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