Cargando…

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

CRISPR-Cas9-based genetic screens are a powerful new tool in biology. By simply altering the sequence of the single-guide RNA (sgRNA), Cas9 can be reprogrammed to target different sites in the genome with relative ease, but the on-target activity and off-target effects of individual sgRNAs can vary...

Descripción completa

Detalles Bibliográficos
Autores principales:	Doench, John G., Fusi, Nicolo, Sullender, Meagan, Hegde, Mudra, Vaimberg, Emma W., Donovan, Katherine F., Smith, Ian, Tothova, Zuzana, Wilen, Craig, Orchard, Robert, Virgin, Herbert W., Listgarten, Jennifer, Root, David E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744125/ https://www.ncbi.nlm.nih.gov/pubmed/26780180 http://dx.doi.org/10.1038/nbt.3437

Ejemplares similares

Creation of Novel Protein Variants with CRISPR/Cas9-Mediated Mutagenesis: Turning a Screening By-Product into a Discovery Tool
por: Donovan, Katherine F., et al.
Publicado: (2017)

Orthologous CRISPR-Cas9 enzymes for Combinatorial Genetic Screens
por: Najm, Fadi J, et al.
Publicado: (2017)

Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR screening
por: DeWeirdt, Peter C., et al.
Publicado: (2022)

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities
por: Sanson, Kendall R., et al.
Publicado: (2018)

Sequence determinants of improved CRISPR sgRNA design
por: Xu, Han, et al.
Publicado: (2015)

Uncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screens
por: Hegde, Mudra, et al.
Publicado: (2018)

CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
por: Chylinski, Krzysztof, et al.
Publicado: (2019)

A quick guide to CRISPR sgRNA design tools
por: Brazelton, Vincent A, et al.
Publicado: (2016)

Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors
por: Replogle, Joseph M, et al.
Publicado: (2022)

A systematic evaluation of nucleotide properties for CRISPR sgRNA design
por: Kuan, Pei Fen, et al.
Publicado: (2017)

Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique
por: Zhang, Beibei, et al.
Publicado: (2021)

Evaluation of sgRNA Target Sites for CRISPR-Mediated Repression of TP53
por: Lawhorn, Ingrid E. B., et al.
Publicado: (2014)

Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
por: Dang, Ying, et al.
Publicado: (2015)

Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
por: Zhang, Jian-Ping, et al.
Publicado: (2016)

Engineering Cellular Signal Sensors based on CRISPR-sgRNA Reconstruction Approaches
por: Zhan, Hengji, et al.
Publicado: (2020)

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
por: Long, Lu, et al.
Publicado: (2018)

In silico sgRNA tool design for CRISPR control of quorum sensing in Acinetobacter species
por: Karlapudi, Abraham Peele, et al.
Publicado: (2018)

Genome-scale CRISPR screening at high sensitivity with an empirically designed sgRNA library
por: Henkel, Luisa, et al.
Publicado: (2020)

AttCRISPR: a spacetime interpretable model for prediction of sgRNA on-target activity
por: Xiao, Li-Ming, et al.
Publicado: (2021)

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites
por: Xie, Shengsong, et al.
Publicado: (2014)

TransCRISPR–sgRNA design tool for CRISPR/Cas9 experiments targeting specific sequence motifs
por: Woźniak, Tomasz, et al.
Publicado: (2023)

sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
por: Graf, Robin, et al.
Publicado: (2019)

Targeting N-Terminal Huntingtin with a Dual-sgRNA Strategy by CRISPR/Cas9
por: Wu, Junjiao, et al.
Publicado: (2019)

A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries
por: Yates, Joshua D, et al.
Publicado: (2021)

CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity
por: Carrington, Blake, et al.
Publicado: (2015)

Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans
por: Chen, Xiangyang, et al.
Publicado: (2014)

A Molecular Chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions
por: Cheng, Jijun, et al.
Publicado: (2016)

Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications
por: Labuhn, Maurice, et al.
Publicado: (2018)

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
por: Gee, Peter, et al.
Publicado: (2020)

EpiCas-DL: Predicting sgRNA activity for CRISPR-mediated epigenome editing by deep learning
por: Yang, Qianqian, et al.
Publicado: (2022)

New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference
por: Jeong, Song Hee, et al.
Publicado: (2023)

sgRNA-PSM: Predict sgRNAs On-Target Activity Based on Position-Specific Mismatch
por: Liu, Bin, et al.
Publicado: (2020)

Synthesis of an arrayed sgRNA library targeting the human genome
por: Schmidt, Tobias, et al.
Publicado: (2015)

Prediction of sgRNA on-target activity in bacteria by deep learning
por: Wang, Lei, et al.
Publicado: (2019)

Optimized minimal genome-wide human sgRNA library
por: Zhou, Yangfan, et al.
Publicado: (2023)

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
por: Jiang, Wenzhi, et al.
Publicado: (2013)

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression
por: Radzisheuskaya, Aliaksandra, et al.
Publicado: (2016)

CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication
por: Zhao, Changzhi, et al.
Publicado: (2020)

Dual sgRNA‐directed gene deletion in basidiomycete Ganoderma lucidum using the CRISPR/Cas9 system
por: Liu, Ke, et al.
Publicado: (2020)

C-RNNCrispr: Prediction of CRISPR/Cas9 sgRNA activity using convolutional and recurrent neural networks
por: Zhang, Guishan, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_fp3b7o4msq4rr1efenpbrfm1h7