Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris

BACKGROUND: Multiple cognate gene copy clones have often been used in order to increase the yield of recombinant protein expression in the yeast Pichia pastoris. The method of posttransformational vector amplification (PTVA) has allowed for the efficient generation of multi-copy clones in P. pastori...

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Detalles Bibliográficos
Autores principales: Aw, Rochelle, Polizzi, Karen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744420/
https://www.ncbi.nlm.nih.gov/pubmed/26849882
http://dx.doi.org/10.1186/s12934-016-0432-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4744420/
https://www.ncbi.nlm.nih.gov/pubmed/26849882
http://dx.doi.org/10.1186/s12934-016-0432-8

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