A Rapid and Sensitive Diagnostic Screening Assay for Detection of Mycobacteria Including Mycobacterium tuberculosis Directly from Sputum without Extraction

We report a novel approach utilising a real-time PCR screening assay targeting a 53 bp tandemly repeated element present at various loci within the Mycobacterium tuberculosis (Mtb) genome. Positive samples were identified within a discriminatory melting curve range of 90–94°C, with results obtained...

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Detalles Bibliográficos
Autores principales: Cross, Lisa Jane, Anscombe, Catherine, McHugh, Timothy D., Abubakar, Ibrahim, Shorten, Robert John, Thorne, Nicola, Arnold, Cath
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745473/
https://www.ncbi.nlm.nih.gov/pubmed/26904747
http://dx.doi.org/10.1155/2015/593745

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745473/
https://www.ncbi.nlm.nih.gov/pubmed/26904747
http://dx.doi.org/10.1155/2015/593745

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