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Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts
Maternal immune adaptation is required for a successful pregnancy to avoid rejection of the fetal–placental unit. Dendritic cells within the decidual microenvironment lock in a tolerogenic profile. However, how these tolerogenic DCs are induced and the underlying mechanisms are largely unknown. In t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746586/ https://www.ncbi.nlm.nih.gov/pubmed/26857012 http://dx.doi.org/10.1038/srep20409 |
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author | Zhao, Lei Shao, Qianqian Zhang, Yun Zhang, Lin He, Ying Wang, Lijie Kong, Beihua Qu, Xun |
author_facet | Zhao, Lei Shao, Qianqian Zhang, Yun Zhang, Lin He, Ying Wang, Lijie Kong, Beihua Qu, Xun |
author_sort | Zhao, Lei |
collection | PubMed |
description | Maternal immune adaptation is required for a successful pregnancy to avoid rejection of the fetal–placental unit. Dendritic cells within the decidual microenvironment lock in a tolerogenic profile. However, how these tolerogenic DCs are induced and the underlying mechanisms are largely unknown. In this study, we show that human extravillous trophoblasts redirect the monocyte-to-DC transition and induce regulatory dendritic cells. DCs differentiated from blood monocytes in the presence of human extravillous trophoblast cell line HTR-8/SVneo displayed a DC-SIGN(+)CD14(+)CD1a(−) phenotype, similar with decidual DCs. HTR8-conditioned DCs were unable to develop a fully mature phenotype in response to LPS, and altered the cytokine secretory profile significantly. Functionally, conditioned DCs poorly induced the proliferation and activation of allogeneic T cells, whereas promoted CD4(+)CD25(+)Foxp3(+) Treg cells generation. Furthermore, the supernatant from DC and HTR-8/SVneo coculture system contained significant high amount of M-CSF and MCP-1. Using neutralizing antibodies, we discussed the role of M-CSF and MCP-1 during monocyte-to-DCs differentiation mediated by extravillous trophoblasts. Our data indicate that human extravillous trophoblasts play an important role in modulating the monocyte-to-DC differentiation through M-CSF and MCP-1, which facilitate the establishment of a tolerogenic microenvironment at the maternal–fetal interface. |
format | Online Article Text |
id | pubmed-4746586 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47465862016-02-17 Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts Zhao, Lei Shao, Qianqian Zhang, Yun Zhang, Lin He, Ying Wang, Lijie Kong, Beihua Qu, Xun Sci Rep Article Maternal immune adaptation is required for a successful pregnancy to avoid rejection of the fetal–placental unit. Dendritic cells within the decidual microenvironment lock in a tolerogenic profile. However, how these tolerogenic DCs are induced and the underlying mechanisms are largely unknown. In this study, we show that human extravillous trophoblasts redirect the monocyte-to-DC transition and induce regulatory dendritic cells. DCs differentiated from blood monocytes in the presence of human extravillous trophoblast cell line HTR-8/SVneo displayed a DC-SIGN(+)CD14(+)CD1a(−) phenotype, similar with decidual DCs. HTR8-conditioned DCs were unable to develop a fully mature phenotype in response to LPS, and altered the cytokine secretory profile significantly. Functionally, conditioned DCs poorly induced the proliferation and activation of allogeneic T cells, whereas promoted CD4(+)CD25(+)Foxp3(+) Treg cells generation. Furthermore, the supernatant from DC and HTR-8/SVneo coculture system contained significant high amount of M-CSF and MCP-1. Using neutralizing antibodies, we discussed the role of M-CSF and MCP-1 during monocyte-to-DCs differentiation mediated by extravillous trophoblasts. Our data indicate that human extravillous trophoblasts play an important role in modulating the monocyte-to-DC differentiation through M-CSF and MCP-1, which facilitate the establishment of a tolerogenic microenvironment at the maternal–fetal interface. Nature Publishing Group 2016-02-09 /pmc/articles/PMC4746586/ /pubmed/26857012 http://dx.doi.org/10.1038/srep20409 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhao, Lei Shao, Qianqian Zhang, Yun Zhang, Lin He, Ying Wang, Lijie Kong, Beihua Qu, Xun Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title | Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title_full | Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title_fullStr | Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title_full_unstemmed | Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title_short | Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
title_sort | human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746586/ https://www.ncbi.nlm.nih.gov/pubmed/26857012 http://dx.doi.org/10.1038/srep20409 |
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